2013 Fiscal Year Final Research Report
Roles of COPII-mediated vesicular transport in higher animals
| Project/Area Number |
23570239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
TANI KATSUKO 東京薬科大学, 生命科学部, 教授 (40266896)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 小胞体 / ペルオキシソーム / p125 / Sec16B |
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| Research Abstract |
Coat protein complex II (COPII)-coated vesicles mediate protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. In this study, we analyzed functions of two COPII-interacting proteins, p125 and Sec16B. We found that loss of p125 reduced the interaction between Sec23 (an inner layer component of COPII) and Sec31 (an outer layer component of COPII), suggesting a role in the assembly of COPII. We also demonstrated that Sec16B is involved in the transport of Pex16, a peroxisomal membrane protein, from the ER to peroxisomes. Our results with mutant Sec16B proteins suggest that ER exit sites, which are an ER subdomain where COPII vesicles are formed, are not involved in this process. In addition, we found that Sec16B might be involved in lipid droplet biogenesis in HeLa cells.
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[Journal Article] Phosphatidic acid (PA)- preferring phospholipase A_1 regulates mitochondrial dynamics2014
Author(s)
Baba, T., Kashiwagi, Y., Arimitsu, N., Kogure, T., Edo, A., Maruyama, T., Nakao, K., Nakanishi, H., Kinoshita, M., Frohman, M.A., Yamamoto, A., and Tani, K.
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Journal Title
J.Biol.Chem.
Volume: 289
Pages: 11497-11511
DOI
Peer Reviewed
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[Journal Article] Roles of SAM and DDHD domains in mammalian intracellular phospholipase A_1 KIAA0725p2012
Author(s)
Inoue, H., Baba, T., Sato, S., Ohtsuki, R., Takemori, A., Watanabe, T., Tagaya, M., and Tani, K.
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Journal Title
Biochim Biophys Acta
Volume: 1823
Pages: 930-939
Peer Reviewed
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[Journal Article] Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells2011
Author(s)
Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Ogasawara, Y., Yamamoto, A., Tagaya, M., and Tani, K.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 108
Pages: 12746-12751
DOI
Peer Reviewed
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[Presentation] Sec16B is involved in the ER export of the peroxisomal membrane proteins Pex3 and Pex16 in mammalian cells2011
Author(s)
Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Ogasawara, Y., Yamamoto, A., Tagaya, M., and Tani, K.
Organizer
Annual Meeting of the American Society for Cell Biology
Place of Presentation
Denver, CO, USA
Year and Date
20111200
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[Presentation] Sec16B is involved in the ER export of the peroxisomal membrane biogenesis factor Pex16 in mammalian cells2011
Author(s)
Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Tagaya, M., and Tani, K.
Organizer
第63回日本細胞生物学会大会
Place of Presentation
北海道
Year and Date
20110600
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