2013 Fiscal Year Final Research Report
Mechanism and function of a novel stress-induced nuclear import pathway
| Project/Area Number |
23570242
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KOSE Shingo 独立行政法人理化学研究所, 今本細胞核機能研究室, 専任研究員 (90333278)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 核-細胞質間輸送 / 分子シャペロン / 熱ストレス |
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| Research Abstract |
Nuclear import of molecular chaperones HSP70 is up-regulated during cellular thermal stresses. However, the mechanism of nuclear transport and physiological significance of HSP70 in the nucleus remains to be unknown. Using our in vitro transport system under heat stress conditions, we identified a novel nuclear import carrier named Hikeshi that mediates heat-stress-induced nuclear import of HSP70. Unlike importin-beta-family-mediated nuclear transport, the Hikeshi-mediated nuclear import of HSP70 is coupled with the ATPase cycle of HSP70. In human living cells, depletion of Hikeshi reduces cell viability during recovery from heat stress conditions, and delays the attenuation and reversion of the heat stress response significantly. Nuclear HSP70 rescues the effect of Hikeshi-depletion partially, suggesting that HSP70 counteracts to the heat stress damages by acting in the nucleus.
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[Journal Article] The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts2014
Author(s)
Oda, Y., Kimura, M., Kose, S., Fasken, M.B., Corbett, A.H., Imamoto, N.
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Journal Title
FEBS Lett., pii
Volume: S0014-5793(14)
Pages: 00304-4
DOI
Peer Reviewed
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[Journal Article] Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and SILAC-based quantitative proteomics2013
Author(s)
Kimura, M., Kose, S., Okumura, N., Imai, K., Furuta, M., Sakiyama, N., Tomii, K., Horton, P., Takao, T., and Imamoto, N.
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Journal Title
Mol. Cell. Proteomics
Volume: 12
Pages: 145-157
DOI
Peer Reviewed
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[Presentation] 熱ストレス時に誘導される分子シャペロンHsp70のHikeshi依存的核内移行( Thermal stress-induced nuclear import of Hsp70 mediated)2013
Author(s)
小瀬真吾, 亀高愛, 本橋詳子, 渡邊愛, 今本尚子(理研・今本細胞核機能研究室)( Hikeshi Shingo Kose, Ai Kametaka, Shoko Motohashi, Ai Watanabe, Shoko Motohashi, Naoko Imamoto(Cellular Dynamics Laboratory, RIKEN))
Organizer
第36回日本分子生物学会年会. (The 36th Annual Meeting of the Molecular Biology Society of Japan)
Place of Presentation
神戸市(神戸ポートアイランド)
Year and Date
20131203-06
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[Presentation] 核-細胞質間運搬体分子Hikeshi と分子シャペロンHsp70 の相互作用とその機能解析(Analysis of the interaction betweennuclear import carrier, Hikeshi, andmolecular chaperone Hsp70 and its cellular functionShingo Kose)2013
Author(s)
小瀬真吾, 渡邊愛, 亀高愛, 本橋詳子, 今本尚子. (理研・今本細胞核機能研究室) (Ai Watanabe, Ai Kametaka,Shoko Motohashi, Naoko Imamoto. (Cellular Dynamics Lab., RIKEN))
Organizer
第86回日本生化学会大会
Place of Presentation
横浜市(パシフィコ横浜)
Year and Date
20130911-13
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[Presentation] Nuclear import receptor, Hikeshi, for Hsp70s is required for attenuation of heat-shock response and protecting cell damages from stress2012
Author(s)
Kose, S., Kametaka, A., Watanabe, A., and Imamoto, N.
Organizer
Dynamic Organization of Nuclear Function Meeting, Cold Spring Harbor
Place of Presentation
New York, USA
Year and Date
20120927-1001
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