2013 Fiscal Year Final Research Report

Elucidation of polarity mechanism in migrating cells and analysis of molecular dynamics of phospholipase D on the cell membrane

Research Project

Project/Area Number	23570244
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Cell biology
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	NAGASAKI Akira 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (30392640)
Project Period (FY)	2011 – 2013
Keywords	PLD / 細胞運動 / 全反射顕微鏡 / NBT-II
Research Abstract	Cell migration plays a major role in a variety of normal biological processes and metastasis of cancer cells. It is known that cell migration is controlled by several cell events (e.g., regulation of actin-cytoskeleton, substrate adhesion, membrane traffic, and cell polarity). As a consequence, the system regulating cell migration is very complicated, requiring coordinated spatiotemporal control of these cell events during the migration process. Hitherto, we screened genes regulating cell migration by using our screening system, and PLD and 32 kinase-related genes were identified. In this study, I investigated the single molecule dynamics and activation of PLD on cell membrane by visualization of PLD and its products, phosphatidic acid. Furthermore, I performed pathway analysis among identified gene products to construct a comprehensive signal network of cell migration.