2013 Fiscal Year Final Research Report
Role of Sox17 family proteins in hematopoiesis at midgestation of mouse embryos
| Project/Area Number |
23570250
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NOBUHISA Ikuo 東京医科歯科大学, 難治疾患研究所, 准教授 (40332879)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Sox17 / AGM / hematopoiesis |
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| Research Abstract |
During mouse development, definitive hematopoiesis is first detected in the aorta-gonad-mesonephros (AGM) region at around embryonic day 10.5 (E10.5), which exhibits intra-aortic cell clusters (IACCs). These clusters are known to contain hematopoietic stem cells (HSCs). It is unclear how the cells in such clusters maintain their HSC phenotype. We found that a transcription factor, Sox17, and other F-group (SoxF) proteins, Sox7 and Sox18, were expressed in E10.5 IACCs. Overexpression of any of these SoxF proteins in IACCs led to consistent formation of cell clusters in vitro during several passages of cocultures with stromal cells. Sox17-transduced cells retained a long-term bone marrow reconstitution activity in vivo. Shutdown of exogenously introduced Sox17 gene expression resulted in immediate hematopoietic differentiation. These results indicate that SoxF proteins, especially Sox17, contribute to the maintenance of cell clusters containing HSCs in the midgestation AGM region.
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[Journal Article] Sox17 haploinsufficiency results in perinatal biliary atresia and hepatitis in C57BL/6 background mice2013
Author(s)
Uemura M, Ozawa A, Nagata T, Kurasawa K, Tsunekawa N, Nobuhisa I, Taga T, Hara K, Kudo A, Kawakami H, Saijoh Y, Kurohmaru M, Kanai Azuma M, and Kanai Y
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Journal Title
Development
Volume: 140(3)
Pages: 639-48
DOI
Peer Reviewed
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[Journal Article] Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters
Author(s)
Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takaki H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T
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Journal Title
Mol Cell Biol
Volume: (in press)
Peer Reviewed
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[Presentation] Introduction of Sox17 in the aorta-gonad-mesonephros-derived hematopoietic cells leads to increased common myeloid progenitors in transplanted mice2013
Author(s)
信久幾夫, 大澤光次郎, 上村麻実, 原田果歩, Maha Anani, 齋藤清香, 高木春奈, 金井正美, 金井克晃, 岩間厚志, 田賀哲也
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸
Year and Date
20131203-06
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[Presentation] Sox17 protein-mediated maintenance of cells with stem cell phenotype in the hematopoietic cell clusters in the fetal AGM region2013
Author(s)
信久幾夫, 大澤光次郎, 上村麻実, 岸川陽子, Maha Anani, 原田果歩, 高木春奈, 金井正美, 金井克晃, 岩間厚志, 田賀哲也
Organizer
第11回幹細胞シンポジウム
Place of Presentation
東京
Year and Date
20130517-18
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[Presentation] Hematopoietic cell clusters present in the aorta-gonad-mesonephros region exhibited long-term repopulating ability by Sox17-expression2012
Author(s)
信久幾夫, 大澤光次郎, 上村麻実, 岸川陽子, Maha Anani, 原田果歩, 高木春奈, 金井正美, 金井克晃, 岩間厚志, 田賀哲也
Organizer
第35回日本分子生物学会年会
Place of Presentation
神戸
Year and Date
20121211-14
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[Presentation] SOX-F FAMILY PROTEINS HAVE ROLES IN THE MAINTAINANCE OF IMMATURE PHENOTYPE OF THE HEMATOPOIETIC CELL CLUSTERS IN THE AORTA-GONAD-MESONEPHROS REGION OF MOUSE EMBRYOS2012
Author(s)
信久幾, 大澤光次郎, 上村麻実, 岸川陽子, Maha Anani, 原田果歩, 高木春奈, 宮東明彦, 金井正美, 金井克晃, 岩間厚志, 田賀哲也
Organizer
The 10^<th> Annual Meeting of International Society for Stem Cell Research
Place of Presentation
横浜
Year and Date
20120613-16
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