2013 Fiscal Year Final Research Report
Alteration in Cell-cell and cell-ECM interaction triggers amphibian retinal regeneration
| Project/Area Number |
23570255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nara Women's University |
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Principal Investigator |
ARAKI Masasuke 奈良女子大学, 自然科学系, 教授 (00118449)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 網膜再生 / ツメガエル / 網膜色素上皮細胞 / 細胞結合 |
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| Research Abstract |
In the amphibian retinal regeneration RPE cells play a critical role that RPE cells transdifferentiate into retinal cells. Immediate after retinal removal, RPE cells detach from the basement membrane and migrate to the retinal vascular membrane, where RPE cells form an epithelial sheet. Using a tissue culture model we have analyzed the expression of Rx and Pax6, essential genes to retinal development, in RPE cells how it is related to the alteration of cell-cell and cell-extracellular matrix interaction. Expression of Rx appears to be up-regulated by the detachment of RPE cells from the basement membrane, while expression of Pax6 was observed in cells without gap junction. Cell proliferation was also observed in the RPE cells that have lost gap junction. The initial detachment of RPE cells from the basement membrane appears to be stimulated by the expression of Matrix metalloproteinase (MMPs), since the suppression of MMPs does not induce transdifferentiation.
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