Molecular network and functional analysis of gonad specific microRNA

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: TAKADA Shuji 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 部長 (20382856)
• Project Period (FY): 2011 – 2013
• Keywords: microRNA / セルトリ細胞 / フォリキュラー細胞 / 性分化

Research Abstract

In order to analyze functions of microRNA in gonadogenesis, functions of microRNA-202, which is expressed specifically in gonads, were studied. Since microRNA functions through target mRNA, a target of microRNA-202 was screened by luciferase reporter assay. Also microRNA-202 KO mouse was established by genome editing utilizing TALEN system. The microRNA-202 KO mouse did not show abnormality by macroscopic specification.

Research Products

• [Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection (2013)
  • Author(s): Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S
  • Journal Title: Sci Rep Volume: 3 Pages: 3136
  • DOI: 10.1038/srep03136
  • Peer Reviewed
• [Journal Article] Targeted gene deletion of miRNAs in mice by TALEN system (2013)
  • Author(s): Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Asahara H
  • Journal Title: PLoS One Volume: 8(10)
  • DOI: 10.1371/journal.pone.0076004
  • Peer Reviewed
• [Presentation] TALEN技術を用いたSry KOマウスの作出 (2013)
  • Author(s): 加藤朋子,宮田康平,園部未来,山下聡,玉野萌恵,宮本新吾,佐久間哲史,山本卓,乾雅史,菊水健史,浅原弘嗣,高田修治
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2013-12-05
• [Presentation] SOX9はオス生殖腺においてDhhの発現を直接制御する (2013)
  • Author(s): 山下聡,加藤朋子,山口勝司,重信秀治,乾雅史,高田修治,浅原弘嗣
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2013-12-05
• [Presentation] ハイスループット・スクリーニング系を用いた卵巣形成関連候補因子の制御因子の探索 (2013)
  • Author(s): 乾雅史,加藤朋子,五十嵐ありさ,浅原弘嗣,高田修治
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2013-12-05
• [Presentation] 生殖腺特異的マイクロRNAの同定と機能解析 Identification and characterisation of gonad-specific microRNA (2012)
  • Author(s): 高田修治
  • Organizer: 第35回日本分子生物学会年会 :ワークショップ「哺乳類の性分化・性成熟の新知見:性腺機能を制御する遺伝子ネットワークとその破綻による疾患」
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-12-13
• [Presentation] MicroRNA-202 is specifically expressed in the gonads through early to terminal differentiation stages in the mouse (2012)
  • Author(s): Shuji Takada
  • Organizer: Sixth International Symposium on The Biology of Vertebrate Sex Determination
  • Place of Presentation: King Kamehameha's Kona Beach Hotel(Hawaii、USA)
  • Year and Date: 2012-04-25
• [Presentation] microRNA-202は生殖腺特異的に生殖腺の分化初期から発現する (2011)
  • Author(s): 高田修治,渡辺義隆,久貝朋子,玉野萌恵,五十嵐ありさ,浅原弘嗣
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-13