2013 Fiscal Year Final Research Report
Reduction of oxidative stress by dietary restriction: reacquiring of the functional potentials
| Project/Area Number |
23570290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied anthropology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KOHNO hirao 関西医科大学, 医学部, 講師 (30148522)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 食事制限 / 酸化ストレス |
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| Research Abstract |
To elucidate the molecular basis of stress-resistance induced by dietary restriction, an in vitro evaluating system for the effects of nutrient-deficiency on the induction of oxidative-stress related genes was established in this study. The expression of metallothionein-Ia (MT-Ia) gene in human hepatoma cells was significantly enhanced by culture in media which lack either fetal calf serum, or cysteine. The treatment with an inhibitor of glycolysis, 2-deoxyglucose, also induced the expression of MT-Ia gene. From these results, it was suggested that human cells have potential to resist against oxidative stress by three different mechanisms, which could be reacquired by dietary restriction.
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