2013 Fiscal Year Final Research Report
Positional cloning and functional analysis of CR-genes in Brassica oleracea
| Project/Area Number |
23580003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 根こぶ病 / Brassica oleracea / 病害抵抗性遺伝子 |
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| Research Abstract |
In order to isolate clubroot resistance gene (CR-gene) in cabbage, we mapped 30 markers that can detected polymorphism in CR-gene regions of the susceptible and resistance parents. The recombinant plants, which had crossovers in the CR-gene region, were genotyped using our designed markers to obtain graph genotypes. The inoculation to the graph genotypes revealed that the CR-gene region is located around the KBr marker. We determined the DNA sequence of the CR-gene region from next-generation sequencing analysis of the susceptible parent resistance. The candidate gene region will be sub-cloned from BAC and cosmid clones, already cloned and contained the target region.
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