2013 Fiscal Year Final Research Report
Molecular cytological study for ASGR-carrier chromosome in aposporous plant
| Project/Area Number |
23580011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
AKIYAMA Yukio 独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター畜産飼料作研究領域, 主任研究員 (70403160)
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| Co-Investigator(Renkei-kenkyūsha) |
OZIAS Akins, Peggy ジョージア大学, 園芸学部, 教授
EBINA Masumi 独立行政法人農業食品産業技術総合研究機構, 畜産草地研究所・飼料作物研究領域, 上席研究員 (80355122)
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| Project Period (FY) |
2011 – 2013
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| Keywords | アポミクシス / アポスポリー / 染色体 / FISH / 蛍光 in situ ハイブリダイゼーション / ギニアグラス |
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| Research Abstract |
Apomixis is a phenomenon that produces clonal progeny through seed reproduction. Apospory is a mode of apomixis that is considered to be controlled by the Apospory-Specific Genomic Region (ASGR)-carrier chromosome. The ASGR-carrier chromosome was discovered in aposporous Pennisetum and Cenchrus species, but a portion of it (the ASGR) is absent from related sexual species. The ASGR-carrier chromosome was also discovered in Guineagrass (Panicum maximum).
In this study, I aimed to analyze the genomic position of three expressed sequence tags (ESTs) reported as candidate genes for apospory and to compare the ASGR between P. maximum and Pennisetum species. The results indicated that the ESTs were not mapped in the ASGR, therefore, the ESTs were probably not responsible genes for apospory, and that the ASGR was conserved between P. maximum and Pennisetum species, supporting the hypothesis "Apospory was spread by transmission of the ASGR.".
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