2013 Fiscal Year Final Research Report
Analysis on allergenic 13S globulin subunits of buckwheat
Project/Area Number |
23580020
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Crop science/Weed science
|
Research Institution | Kyoto University |
Principal Investigator |
TANAKA Tomoyuki (勝部 朋之) 京都大学, (連合)農学研究科(研究院), 准教授 (50224473)
|
Project Period (FY) |
2011 – 2013
|
Keywords | ソバ / 13Sグロブリン / 反復配列 |
Research Abstract |
Buckwheat genomic DNA library, consisting of 142,005 clones, was screened to elucidate the diversity of 13S globulin genes. Twenty-six BAC clones were isolated and 17 genes of 13S globulin were identified, the sequences of which were deposited into DNA Data Bank of Japan. The unit length of nucleotides for most tandem repeat sequences, which are specifically inserted into alpha polypeptides of 13S globulin, was 45 bp while that of some repeats had extra three nucleotides like CGG or AGA, resulting in different secondary structure formation. On the other hand, preliminary experimental system was developed to analyze the effect of environmental factors on 13S globulin composition using autogamous tartary buckwheat.
|
-
-
[Journal Article] Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat2014
Author(s)
Sano, M., Nakagawa, M., Oishi, A., Yasui, Y., Katsube-Tanaka, T
-
Journal Title
Food Chemistry
Volume: 155
Pages: 192–198
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-