2013 Fiscal Year Final Research Report
Development of novel breeding procedure by using plant regeneration system from endosperm culture
| Project/Area Number |
23580029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Hokkaido University |
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Principal Investigator |
HOSHINO YOICHIRO 北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 胚乳 / ハスカップ |
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| Research Abstract |
Plant regeneration system from endosperm culture has been developed in Haskap (Lonicera caerulea var. emphyllocalyx). The plants derived from endosperm showed same ploidy level with endosperm. This means that endosperm culture is useful for novel breeding procedure. To analyze process of endosperm culture, full length of fertilization independent endosperm (FIE) homolog was sequenced in Haskap. Expression pattern of FIE homolog was analyzed by real-time PCR during endosperm development in vivo and endosperm culture in vitro. The process of endosperm culture was discussed in expression pattern of FIE homolog.
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