2013 Fiscal Year Final Research Report
Genetic analysis for alteration of essential oil fragrance in lavender
| Project/Area Number |
23580053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Meijo University |
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Principal Investigator |
TSURO MASATO 名城大学, 農学部, 准教授 (40410774)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMIYAMA Yasuhiro 和歌山大学, 教育学部, 准教授 (00463266)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ラベンダー / 精油 / モノテルペン / SSR / 香り |
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| Research Abstract |
In order to alter the fragrance of essential oil of lavender, we conducted two major works; 1) construction of the SSR marker, and 2) production of transgenic plants with limonene synthase gene. The results of these works are as follows. 1) Eleven primer pairs of SSR markers were constructed. There markers could distinguish species among section lavandula, while only a few of them could be available to identify other species except for section lavandula. 2) In transgenic plants with 35S-driven limonene synthase gene (LIMS), overexpressed LIMS increased not only limonene but also other cyclic monoterpenoid production in vegetative leaves, while suppression of LIMS expression reduced their total essential oil production, including the dramatic decrease of limonene, linalool, and linalyl acetate, in florets of the reproductive stage. These results suggested that transgene expression differed by organs.
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