2013 Fiscal Year Final Research Report
Structural based fruit softening mechanism; the model of tomato fruit softening during maturation
| Project/Area Number |
23580054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 果実軟化 / 細胞壁 / トマト果実 / β-ガラクトシダーゼ |
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| Research Abstract |
In tomato fruit, beta-galactosidase 4 (TBG4) is an enzyme responsible for fruit softening through the degradation of beta-(1,4)-galactan in the pericarp cell wall. To gain structural insight into the substrate specificity, we determined the crystal structures of TBG4 and its complex with beta-D-galactose. TBG4 was composed of a catalytic TIM barrel domain followed by three beta-sandwich domains. This structure is similar to other beta-galactosidase belong to GH35 families.
In addition, we determined the enzymatic properties and substrate specificities of TBG1. TBG4 has substrate recognition to beta-(1,4)-linkage, however, TBG1 has beta-(1,3) and beta-(1,6)-likage substrate.
In these results, TBG's act on the tomato cell wall during fruits development and maturation, and seberal stage of tomato fruits cell walls construct/reconstruct to some components sugar as a substrate against TBG's.
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Research Products
(6 results)
