Elucidation of the mechanisme of specific recognition between rice and rice blast

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: KANZAKI Hiroyuki 公益財団法人岩手生物工学研究センター, 生命科学研究部, 研究専門員 (10390882)
• Project Period (FY): 2011 – 2013
• Keywords: イネ / いもち病菌 / 非病原力遺伝子AVR-Pik / 認識特異性 / タンパク質相互作用 / Dual抵抗性遺伝子

Research Abstract

Between pathogen and host, attack and counter-attack impose strong reciprocal selection on the involved organisms, leading to the development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik and cognate rice resistance (R-) gene Pik are highly variable with multiple alleles differing by DNA replacements causing amino acid changes. There is a tight recognition specificity of AVR-Pik alleles by different Pik alleles. We found that AVR-Pik physically binds the N-terminal coiled-coil (CC) domain of Pik in yeast 2-hybrid assay as well as in in-planta co-immunoprecipitation assay. This binding specificity correlates the recognition specificity between AVR and R-genes. We propose that AVR-Pik and Pik are locked up in arms race co-evolution that is driven by their direct physical interactions.

Research Products

• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Césari, S., Kanzaki, H., Fujiwara, T.(co-first authors), Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds P., Terauchi, R., Kroj, T
  • Journal Title: EMBO journal Volume: (in press)
• [Journal Article] MutMap+: Genetic Mapping and Mutant Identification without Crossing in Rice (2013)
  • Author(s): Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., Yaegashi, H., Sharma, S., Sharma, S., Kanzaki, H., Matsumura, H., Saitoh, H., Mitsuoka, C., Utsushi, H., Uemura, A., Kanzaki, E., Kosugi, S., Yoshida, K., Cano, L., Kamoun, S., Terauchi, R
  • Journal Title: PLoS ONE Volume: 8 Pages: e6852
  • DOI: 10.1371/journal.pone.0068529
• [Journal Article] MutMap-Gap : whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii (2013)
  • Author(s): Takagi H., Uemura A., Yaegashi H., Tamiru M., Abe A., Mitsuoka C., Utsushi H., Natsume S., Kanzaki H., Matsumura H., Saitoh H., Yoshida K., Cano L.M., Kamoun S., Terauchi R
  • Journal Title: New Phytologist Volume: 200 Pages: 276-83
  • DOI: 10.1111/nph.12369.Epub
• [Journal Article] The rice resistance protein pair RGA4/RGA5 recognize the Magnaporthe oryzae effectors AVR-Pia and AVR-CO39 by direct binding (2013)
  • Author(s): Cesari, S., Thilliez, G., Ribot, C., Chalvon, V., Michel, C., Jauneau, A., Rivas, S., Alaux, L., Kanzaki, H., Okuyama, Y., Morel, J.B., Fournier, E., Terauchi, R., Kroj, T
  • Journal Title: Plant Cell Volume: 25 Pages: 1463-1481
  • DOI: 10.1105/tpc.112.107201
• [Journal Article] Mutation in OsLMS, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.) (2012)
  • Author(s): Undan, JR., Tamiru, M., Abe, A., Yoshida, K., Kosugi, S., Takagi, H., Yoshida, K., Kanzaki, H., Saitoh, H., Fekih, R., Sharma, S., Undan, J., Yano, M., Terauchi, R
  • Journal Title: Gene and Genetic Systems Volume: 87 Pages: 169-179
  • DOI: 10.1266/ggs.87.169
• [Journal Article] Whole genome sequencing and future breeding of rice (2012)
  • Author(s): Terauchi, R., Abe, A., Takagi, H., Yoshida, K., Kosugi, S., Natsume, S., Yaegashi, H, Kanzaki, H., Matsumura, H., Mitsuoka, C., Utsushi, H., Tamiru, M
  • Journal Title: Journal of Plant Biochem. Biotechnol Volume: 21 Pages: 10-14
  • DOI: 10.1007/s13562-012-0133-2
• [Journal Article] Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions (2012)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Fujisaki, K., Hirabuchi, A., Alaux, L., Fournier, E., Tharreau, D., Terauchi, R
  • Journal Title: Plant Journal Volume: 72 Pages: 894-907
  • DOI: 10.1111/j.1365-313X.2012.05110.x
• [Journal Article] Genome sequencing reveals agronomically important loci in rice using MutMap (2012)
  • Author(s): Abe, A., Kosugi, S., Yoshida, K., Natsume, S., Takagi, H., Kanzaki, H., Matsumura, H., Yoshida, K., Mitsuoka, C., Tamiru, M., Innan, H., Cano L., Kamoun, S.& Terauchi, R
  • Journal Title: Nature Biotechnology Volume: 30 Pages: 174-178
  • DOI: 10.1038/nbt.2095
• [Journal Article] A multifaceted genomics approach allows the isolation of the rice Pia- blast resistance gene consisting of two adjacent NBS- LRR protein genes (2011)
  • Author(s): Okuyama, Y., Kanzaki, H.(co-first authors), Abe, A., Yoshida K., Tamiru M., Saitoh H., Fujibe T., Matsumura H., Shenton M., Galam, D.C., Undan, J., Ito, A., Sone, T. and Terauchi, R
  • Journal Title: Plant Journal Volume: 66 Pages: 467-479
  • DOI: 10.1111/j.1365-313X.2011.04502.x
• [Presentation] Mark Banfield、Sophien Kamoun、寺内良平いもち病菌(Magnaporthe oryzae)非病原力遺伝子AVR-Pikと相互作用するイネタンパク質の同定 (2014)
  • Author(s): 神崎洋之、斉藤宏昌、藤崎恒喜、小林光智衣、伊藤和江、神崎英子、三岡周子
  • Organizer: 平成26年度日本植物生理学会
  • Place of Presentation: 富山市
  • Year and Date: 20140318-20
• [Presentation] Rice proteins that interact with the M. oryzae avirulence effector Avr-Pik (2013)
  • Author(s): Kanzaki, H., Sautoh, H., Fujisaki, K., Kobayashi, M., Ito, K., Kanzaki, E., Mitsuoka, C., Banfield, M., Kamoun, S., Terauchi, R
  • Organizer: 2013-International Rice Blast Conference
  • Place of Presentation: 韓国済州島
  • Year and Date: 2013-08-20
• [Presentation] Arms race co-evolution of Magnaporthe oryzae AVR gene and rice R genes driven by their physical interactions (2012)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Fujisaki, K., Hirabuchi, A., Allaux, L., Fournier. E., Tharreau. D., Terauchi, R
  • Organizer: 30th New Phytologist Symposium～Immunomodulation by plant-associated organisms
  • Place of Presentation: California米国
  • Year and Date: 20120916-19
• [Presentation] The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik (2012)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Hirabuchi, A., Allaux, L., Fournier. E., Tharreau. D., Terauchi, R
  • Organizer: XI International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都市
  • Year and Date: 20120729-0802
• [Presentation] いもち病菌非病原力遺伝子AVR-Pikが認識する新規イネ遺伝子の単離 (2012)
  • Author(s): 神崎洋之・斉藤宏昌・藤崎恒喜・寺内良平
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
  • Year and Date: 20120327-29
• [Presentation] いもち病菌非病原力遺伝子AVR- Pikとイネいもち病抵抗性遺伝子Pikは、直接的タンパク質相互作用によって認識する (2012)
  • Author(s): 神崎洋之、吉田健太郎、平渕亜紀子、寺内良平
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-03-29
• [Presentation] いもち病菌非病原力遺伝子AVR- Pikとイネいもち病抵抗性遺伝子Pikにおけるアリル間認識特異性 (2011)
  • Author(s): 神崎洋之、吉田健太郎、寺内良平
  • Organizer: 日本育種学会第120回講演会秋季大会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-09-23
• [Book] Plant Immunity (Springer)DOI : 10.1007/978-1-62703-986-4_20 (2013)
  • Author(s): Kanzaki. H., Yoshida, K., Saitoh, H., Tamiru, M. Terauchi, R. (Edit. Baul Birch)
  • Publisher: Protoplast cell death assay to study AVR function in rice (in Methods in Molecular Biology