Resistance mechanisms of the diamondback moth against various insecticides

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Okayama University
• Principal Investigator: SONODA Shoji 岡山大学, 資源植物科学研究所, 准教授 (00325127)
• Project Period (FY): 2011 – 2013
• Keywords: アセチルコリンエステラーゼ / コナガ / 遺伝子重複 / アミノ酸変異 / 有機リン剤

Research Abstract

This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the fieldcollected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P. xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P. xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

Research Products

• [Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate (2014)
  • Author(s): Sonoda, S., Shi, X., Song, D., Liang, P., Gao, X., Zhang, Y., Li, J., Liu, Y., Li, M., Matsumura, M., Sanada-Morimura, S., Minakuchi, C., Tanaka, T., Miyata, T
  • Journal Title: Insect Biochem. Mol. Biol Volume: 48 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance (2012)
  • Author(s): Sonoda, S., Shi, X., Song, D., Zhang, Y., Li J., Wu, G., Liu, Y., Li, M., Liang, P., Wari, D., Matsumura, M., Minakuchi, C., Tanaka, T., Miyata, T., Gao, X
  • Journal Title: Pestic. Biochem. Physiol. Volume: 102 Pages: 102-105
  • Peer Reviewed
• [Presentation] コナガにおけるAChE遺伝子の重複と有機リン剤抵抗性 (2014)
  • Author(s): 園田昌司
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知大学
  • Year and Date: 2014-03-29
• [Presentation] 殺虫剤の作用機作と抵抗性のメカニズム (2013)
  • Author(s): 園田昌司
  • Organizer: 長野県病害虫防除シンポジウム
  • Place of Presentation: 松本市
  • Year and Date: 2013-02-22
• [Presentation] コナガのピレスロイド剤抵抗性-野外におけるナトリウムチャネルのアミノ酸変異 (2012)
  • Author(s): 園田昌司
  • Organizer: NIASシンポジウム「ポストゲノム時代の害虫防除研究のあり方」,第5回-殺虫剤抵抗性問題の最前線
  • Place of Presentation: 東京都
  • Year and Date: 2012-11-15
• [Presentation] 難防除害虫の薬剤抵抗性メカニズム (2012)
  • Author(s): 園田昌司
  • Organizer: 革新的農業技術習得支援事業(野菜の難防除害虫のIPM技術)
  • Place of Presentation: 津市
  • Year and Date: 2012-10-25
• [Presentation] コナガの殺虫剤に対する抵抗性および適応機構 (2012)
  • Author(s): 園田昌司
  • Organizer: 九州昆虫セミナー
  • Place of Presentation: 合志市
  • Year and Date: 2012-10-22
• [Presentation] Organophosphate resistance in the laboratory and field strains of the diamondback moth (2012)
  • Author(s): Sonoda, S
  • Organizer: International Seminar on the Development of Insecticide Resistance and Its Management in the Diamondback Moth
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2012-08-27
• [Presentation] Frequencies of the M918I, T929I and L1014F mutations in the sodium channel of the diamondback moth in China, Thailand and Japan (2012)
  • Author(s): Sonoda, S, et al.
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2012-08-20
• [Presentation] コナガの合成ピレスロイド剤抵抗性に関わるナトリウムチャネルのアミノ酸変異の頻度-日本、中国、タイ系統の比較 (2012)
  • Author(s): 園田昌司, et al
  • Organizer: 日本農薬学会大会第37回大
  • Place of Presentation: 岡山大学
  • Year and Date: 2012-03-15
• [Presentation] Sodium channel mutations associated with pyrethroid resistance and their frequencies in the field strains of the diamondback moth, Plutella xylostella collected at different Asian countries (2011)
  • Author(s): Sonoda, S
  • Organizer: International Symposium on Insecticide Resistance Management
  • Place of Presentation: Guiyamg, China
  • Year and Date: 2011-09-20
• [Book] 植物防疫, 殺虫剤抵抗性機構の解析と今後の課題 (2012)
  • Author(s): 園田昌司
  • Total Pages: 40-45, 66