2013 Fiscal Year Final Research Report
Analysis of dsRNA mechanism of response in insect cell
| Project/Area Number |
23580077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyushu University |
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Principal Investigator |
LEE JAEMAN 九州大学, (連合)農学研究科(研究院), 助教 (50404083)
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| Co-Investigator(Kenkyū-buntansha) |
日下部 宜宏 九州大学, 大学院農学研究院, 教授 (30253595)
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| Project Period (FY) |
2011 – 2013
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| Keywords | RNA interference / 遺伝子機能阻害 / dsRNA応答機構 |
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| Research Abstract |
In order to establish efficient RNA interference induction system in insect cells, we introduced several membrane proteins, which have the ability to uptake dsRNA into the cells. Also the cellular responses against dsRNA uptake were analyzed using silkworm cells. As a result, we found that C. elegans Sid-1 membrane protein possessed an ability to uptake dsRNA efficiently. Using this protein, several transgenic insect cell lines were successfully established. Furthermore, almost all RNAi related components of silkworm were isolated and characterized.
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[Journal Article] Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm2013
Author(s)
Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Hiroaki Mon, Kazuhiro Iiyama, JaeMan Lee, Qingyou Xia, Takahiro Kusakabe
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Journal Title
J. Insect Biotechnol. Seric
Volume: 82
Pages: 19-23
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