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2013 Fiscal Year Final Research Report

Exploration of molecular mechanisms underlying the interaction between host insects and symbiotic bacteria and development of basic technology for the application

Research Project

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Project/Area Number 23580084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

ANBUTSU Hisashi  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30392583)

Co-Investigator(Renkei-kenkyūsha) NIKOH Naruo  放送大学, 教養学部, 教授 (70364916)
Project Period (FY) 2011 – 2013
Keywords共生細菌 / ショウジョウバエ / スピロプラズマ / 生殖操作 / 雄殺し / バクテリオファージ
Research Abstract

Some spiroplasmas cause female-biased sex ratios of their host insects including Drosophila fruit flies, as a result of selective death of the male offspring during embryogenesis. In this study, three closely related bacteriophages were isolated from two male-killing spiroplasmas and a non-male-killing one, and their whole genome sequences were determined. Each phage genome included five sequences with similarity to putative virulence genes of closely related spiroplasma. In general, density dynamics of the three phages showed different pattern. The expression levels of a gene were about 10 times higher in adult flies infected with male-killing spiroplasma than in those infected with non-male-killing one.

  • Research Products

    (7 results)

All 2014 2012 Other

All Journal Article (1 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway2014

    • Author(s)
      Toshiyuki Harumoto, Hisashi Anbutsu, Takema Fukatsu
    • Journal Title

      PLos Pathogen

      Volume: Vol.10 Pages: e1003956

    • DOI

      10.1371/journal.ppat.1003956

  • [Presentation] Quantitative analysis of proliferation and gene expression of bacteriophages infecting male-killing and non-male-killing spiroplasmas in Drosophila2014

    • Author(s)
      Hisashi Anbutsu, Naruo Nikoh, Kohjiro Tanaka, Toshiyuki Harumoto, Takema Fukatsu
    • Organizer
      8th International Wolbachia Conference
    • Place of Presentation
      Innsbruck (Austria)
    • Year and Date
      20140606-11
  • [Presentation] ショウジョウバエ-スピロプラズマ共生系における宿主-共生細菌間相互作用2014

    • Author(s)
      安佛尚志、春本敏之、二河成男、田中康次郎、深津武馬
    • Organizer
      第41回日本マイコプラズマ学会学術集会
    • Place of Presentation
      東京大学伊藤謝恩ホール
    • Year and Date
      2014-05-22
  • [Presentation] ショウジョウバエの共生細菌スピロプラズマのファージの比較ゲノム解析2014

    • Author(s)
      安佛尚志、二河成男、田中康次郎、春本敏之、深津武馬
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学(高知市)
    • Year and Date
      2014-03-27
  • [Presentation] Exploration of host genes involved in Drosophila-Spiroplasma endosymbiosis and male-killing phenotype by using RNA-seq2012

    • Author(s)
      安佛尚志、春本敏之、重信秀治、吉武和敏、深津武馬
    • Organizer
      第10回日本ショウジョウバエ研究会
    • Place of Presentation
      東京慈恵医科大学(新橋)
    • Year and Date
      2012-10-15
  • [Presentation] Comparative genomic analysis of bacteriophages associated with male-killing and non-male-killing spiroplasmas in Drosophila2012

    • Author(s)
      Hisashi Anbutsu, Naruo Nikoh, Kohjiro Tanaka, Takema Fukatsu
    • Organizer
      7th International Wolbachia Conference
    • Place of Presentation
      Oleron Island (France)
    • Year and Date
      2012-06-08
  • [Remarks] 産総研論文検索システム(産総研リポジトリ)

    • URL

      http://www.aist.go.jp/aist_j/aist_repository/index.html

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Published: 2015-07-16  

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