2013 Fiscal Year Final Research Report
Exploration of molecular mechanisms underlying the interaction between host insects and symbiotic bacteria and development of basic technology for the application
| Project/Area Number |
23580084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
ANBUTSU Hisashi 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30392583)
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| Co-Investigator(Renkei-kenkyūsha) |
NIKOH Naruo 放送大学, 教養学部, 教授 (70364916)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 共生細菌 / ショウジョウバエ / スピロプラズマ / 生殖操作 / 雄殺し / バクテリオファージ |
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| Research Abstract |
Some spiroplasmas cause female-biased sex ratios of their host insects including Drosophila fruit flies, as a result of selective death of the male offspring during embryogenesis. In this study, three closely related bacteriophages were isolated from two male-killing spiroplasmas and a non-male-killing one, and their whole genome sequences were determined. Each phage genome included five sequences with similarity to putative virulence genes of closely related spiroplasma. In general, density dynamics of the three phages showed different pattern. The expression levels of a gene were about 10 times higher in adult flies infected with male-killing spiroplasma than in those infected with non-male-killing one.
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