2013 Fiscal Year Final Research Report
Construction of gene expression system using virulent phages.
| Project/Area Number |
23580100
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
KANEKO JUN 東北大学, (連合)農学研究科(研究院), 准教授 (30221188)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Keitaro (独)農業・食品産業技術総合研究機構, 食品総合研究所, 主任研究員 (20353980)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 納豆菌 virulent phages / 感染補助因子 / 遺伝子発現 / 宿主認識 |
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| Research Abstract |
Some Bacillus subtilis natto phages carry gamma-poly glutamate hydrolase gene on their genome, and this gene is strongly expressed during phage lytic cycle. To apply this system for the foreign gene expression system, we determined complete genome sequence of phage phiNIT1 and analyzed the expression system of gamma-PGA hydrolase gene.
As a result, we found that not only phage encoded elements but also both of cis-elements and trans-elements in the bacterium were required for the gene expression. In addition, we identified phage proteins involving in specific recognition of host cell surface.
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