2013 Fiscal Year Final Research Report
Biosynthesis mechanism of a cyclic antibacterial peptide linked at N- and C- terminal amino acids and elucidation of the cyclization significance
| Project/Area Number |
23580101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nihon University (2013)
Tohoku University (2011-2012) |
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Principal Investigator |
KAWAI Yasushi 日本大学, 生物資源科学部, 准教授 (00261496)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Tadao 東北大学, 農学研究科, 教授 (00118358)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Junko 東北大学, 農学研究科, 技術専門職員 (10241556)
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| Project Period (FY) |
2011 – 2013
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| Keywords | バクテリオシン / 抗菌ペプチド / 環状構造 / 乳酸菌 / ガセリ菌 |
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| Research Abstract |
A biosynthesis genes for gassericin A, which was the cyclic antibacterial peptide (bacteriocin) linked at N- and C-termini consisting of 58 amino acid residue, were identified, and gene expression analysis clarified that the leader sequence was important to production of gassericin A not the cyclization. In addition, it was suggested gassericin A was linked at N- and C-terminal amino acids by an ABC-transporter and then secreted to outside the producer cells after cleavage of the leader peptide by a putative novel proteinase.
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[Journal Article] Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA392013
Author(s)
K. NAKAMURA, K. ARAKAWA, Y. KAWAI, N. YASUTA, T. CHUJO, M. WATANABE, H. IIOKA, M. TANIOKA, J. NISHIMURA, H. KITAZAWA, K. TSURUMI and T. SAITO
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Journal Title
Animal Science Journal
Volume: 84
Pages: 144-149
Peer Reviewed
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