2013 Fiscal Year Final Research Report
Arsenic release from paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp. OR-1
| Project/Area Number |
23580103
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Chiba University |
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Principal Investigator |
AMACHI Seigo 千葉大学, 園芸学研究科, 准教授 (80323393)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Noriko (独)農業環境技術研究所, 土壌環境研究領域, 主任研究員 (80345090)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 環境微生物 / ヒ素 / 水田土壌 / 溶出 / Geobacter / ヒ酸還元 / ArrA / XANES |
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| Research Abstract |
Previously, we have succeeded to isolate a novel arsenate-reducing bacterium Geobacter sp. OR-1 from Japanese paddy soil. In this study, we isolated a new arsenate-reducing bacterium Anaeromyxobacter sp. PSR-1 from arsenic-polluted soil. When these bacterial strains were inoculated into sterile paddy soil, arsenic release was reproduced. XANES analysis revealed that these strains were able to reduce arsenate adsorbed on soil solid phase. In addition, the strains could release arsenic adsorbed on ferrihydrite. We also determined draft genome sequences of these strains. Preliminary draft genome analysis of strain OR-1 identified two arsenic islands containing multiple arsA and arsD genes, and possible dissimilatory arsenate-reductase genes (arrAB) were also found.
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