2013 Fiscal Year Final Research Report
Structural and functional analysis and application of decarboxylases catalyzing reverse carboxylation
| Project/Area Number |
23580108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 脱炭酸酵素 / 炭酸固定機能 / 酵素変換 |
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| Research Abstract |
The existence of 2,4-dihydroxybenzoate decarboxylase had been suggested in anaerobic microorganisms. However, microorganisms had not yet be isolated. Therefore, the decarboxylase had not be characterized. In the present study, microorganisms showing 2,4-dihydroxybenzoate decarboxylase activity have been isolated through enrichment culture under semi-anaerobic conditions. The decarboxylase was purified to homogeneity and characterized. In the point of substrate specificity and subunit structure, the present enzyme was different from various hydroxybenzoate decarboxylases reported previously. The reverse carboxylation reaction proceeded regioselectively to form 2,4-dihydroxybenzoate without any by-product.
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