2013 Fiscal Year Final Research Report
Study on yeast mRNA flux in ethanol stress response and during the process of alcoholic fermentation
| Project/Area Number |
23580113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
IZAWA SHINGO 京都工芸繊維大学, 工芸科学研究科, 准教授 (10273517)
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| Project Period (FY) |
2011 – 2013
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| Keywords | P-body / stress granule / mRNA flux / ポリソーム解析 / ストレス / 醸造 / 酵母 |
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| Research Abstract |
We found that unnecessary mRNA is not translated and segregated in P-bodies or stress granules (SGs) in yeast cells under stress conditions of alcoholic fermentation. We also demonstrated several data that indicate the contribution of P-bodies and SGs to effective and selective translation of mRNAs that are really required for the rapid stress response. Additionally, we isolated and characterized several mRNA that were preferentially translated under ethanol stress conditions. Furthermore, we verified that lactic acid can induce P-body formation and translational repression but not SG formation. Effects of acetic acid and propionic acid on mRNP granule formation were different from that of lactic acid.
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