2013 Fiscal Year Final Research Report
The analysis of the de-silencing mechanism in E. coli
| Project/Area Number |
23580114
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
OSHIMA Taku 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50346318)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 微生物遺伝・育種 / 転写制御 / 核様体 / 大腸菌 / サイレンシング |
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| Research Abstract |
The transcriptome analysis with the deletion mutants of hha, ydgT, stpA and hns genes indicated that Hha and YdgT are the proteins which assist H-NS to repress the expression over 100 genes. In addition, lux and lacZ reporter analysis to elucidate the mechanisms of the transcriptional regulations in ybdO and ler promoters indicated that the wide areas in up and downstream of the ybdO promoter is essential for the transcriptional repression of ybdO which depends on H-NS and the derepression of ler promoter with Pch protein observed when the repression of ler genes was cooperatively regulated by H-NS and Hha.
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