2013 Fiscal Year Final Research Report
Roles of cold-inducible chaperones in hyperthermophiles
| Project/Area Number |
23580121
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Kwansei Gakuin University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IMANAKA Tadayuki 立命館大学, 生命科学部, 教授 (30029219)
ATOMI Haruyuki 京都大学, 工学研究科, 教授 (90243047)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | アーキア / 低温ショック / シャペロン / RNAヘリカーゼ |
|---|
| Research Abstract |
It has been suggested that hyperthermophiles are highly primitive microorganisms and that their descendents evolved by adapting to cooler environments. Hence, study of the adaptive mechanisms of hyperthermophiles to cold environments may provide insight into evolutionary processes. In the project, the physiological roles of cold inducible chaperones, cpkA (TK0678) and DEAD box RNA helicase (TK0306) from Thermococcus kodakarensis were investigated, showing that both genes are essential for cell growth at cold stressed environment. In addition, the region required for cold induction of TK0306 was identified. The regulatory element, a five-adenosine sequence has been located in the region between the SD region and the initiation codon (ATG). It seems likely that A5 -dependent cold-induced expression is related to premature termination depending on temperature. This sequence-specific mechanism is unique, and one that has not been identified in other known cold-inducible genes.
|
-
-
-
[Journal Article] Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles2014
Author(s)
Okada, K., Hidese, R., Fukuda, W., Niitsu,M., Takao,K., Horai,Y., Umezawa, N., Higuchi, T., Oshima, T., Yoshikawa, Y., Imanaka, T., Fujiwara, S.
-
Journal Title
J.Bacteriol., (American Society for Microbiology, Washington DC, USA)
Volume: 196/10
Pages: 1866-1876
DOI
Peer Reviewed
-
[Journal Article] Different roles of two transcription factor B proteins in the hyperthermophilic archaeon Thermococcus kodakarensis2014
Author(s)
Hidese, R., Nishikawa, R., Gao, L., Katano, M., Imai, T. Kato, S., Kanai, T., Atomi, H., Imanaka, T., Fujiwara, S.
-
Journal Title
Extremophiles (Springer Co.Ltd., New York)
Volume: 18
Pages: 573-588
DOI
Peer Reviewed
-
[Journal Article] Importance and determinants of induction of cold-induced DEAD RNA helicase in the hyperthermophilic archaeon Thermococcus kodakarensis2013
Author(s)
Nagaoka, E., Hidese, R., Imanaka,T., and Fujiwara, S.
-
Journal Title
J.Bacteriol., (American Society for Microbiology, Washington DC, USA)
Volume: 195/15
Pages: 3442-3450
DOI
Peer Reviewed
-
[Journal Article] A genetic examination of initial amino acid oxidation and glutamate catabolism in the hyperthermophilic archaeon Thermococcus kodakarensis2013
Author(s)
Yokooji, Y., Sato, T., Fujiwara, S., Imanaka, T., and Atomi, H.
-
Journal Title
J.Bacteriol., (American Society for Microbiology, Washington DC, USA)
Volume: 195
Pages: 1940-1948
DOI
Peer Reviewed
-
-
-
[Journal Article] Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases2012
Author(s)
Yasukawa, K., Konishi, A., Shinomura, M., Nagaoka, E., and Fujiwara, S.
-
Journal Title
Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands)
Volume: 427
Pages: 654-658
DOI
Peer Reviewed
-
[Journal Article] Indole-3-glycerol-phosphate synthase is recognized by a cold-inducible group II chaperonin in Thermococcus kodakarensis2012
Author(s)
Gao, L., Danno, A., Fujii, S., Fukuda, W., Imanaka, T., and Fujiwara, S.
-
Journal Title
Appl.Environ. Microbiol. (American Society for Microbiology, Washington DC, USA)
Volume: 78
Pages: 3806-3815
DOI
Peer Reviewed
-
[Journal Article] Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K42012
Author(s)
Sano, S., Yamada, Y., Shinkawa, T., Kato, S., Okada, T., Higashibata, H., and Fujiwara, S.
-
Journal Title
J. Biosci. Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands)
Volume: 113/3
Pages: 315-321
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
