2013 Fiscal Year Final Research Report
Construction of cell-free anammox reaction system
| Project/Area Number |
23580123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Sojo University |
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Principal Investigator |
FUJII Takao 崇城大学, 生物生命学部, 教授 (80165331)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Takashi 崇城大学, 生物生命学部, 准教授 (00425331)
HIRA Daisuke 崇城大学, 生物生命学部, 助教 (00569890)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 嫌気性アンモニア酸化 / 脱窒 / アナモックス / 亜硝酸還元酵素 / ヘムタンパク質 / ヒドラジン合成酵素 / ヒドロキシルアミン酸化還元酵素 |
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| Research Abstract |
This study was performed to construct cell-free anaerobic reaction system by reference to the previously described model for mechanism of anammox. The following important findings for construction of the system were obtained. (1) Hydrazine was synthesized from NO and NH4+ catalyzed by hydrazine synthase with a heterodimer heme protein, NaxLS. (2) An intermediate, NO, was synthesized by Cu-type nitrite reductase (Cu-Nir) in strain KSU-1 although cytochrome cd1 Nir has only been known in other anammox bacteria. (3) A multiheme protein, hydroxylamine oxidoreductase (HAO), catalyzed inter-conversion of NO and hydroxylamine.
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