Definition of species of Gram-negative anaerobic rods by multilocus sequence analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 23580126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAKAMOTO Mitsuo 独立行政法人理化学研究所, バイオリソースセンター, 研究員 (50321766)
• Co-Investigator(Renkei-kenkyūsha): OHKUMA Moriya 独立行政法人理化学研究所, バイオリソースセンター, 室長 (10270597)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: Bacteroides / MLSA / dnaJ / gyrB / hsp60 / recA / rpoB / 16S rRNA

Outline of Final Research Achievements

Multilocus sequence analysis (MLSA) was performed on 107 species of Gram-negative anaerobic rods including the genus Bacteroides. The genes selected, dnaJ, gyrB, hsp60, recA, rpoB and 16S rRNA were amplified by direct PCR and then sequenced. For the genus Bacteroides, we defined species as a group of strains that share at least 97.5 % gene sequence similarity based on the fragments of five protein-coding housekeeping genes and 16S rRNA gene. Analyses of the concatenated sequences of all six genes revealed robust phylogenetic relationships among the species of Gram-negative anaerobic rods when compared with the single-gene trees.

Free Research Field

農学