Study on Evolution and Diversity of Plant Circadian Clocks

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580133
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: TAKAFUMI Yamashino 名古屋大学, 生命農学研究科, 助教 (00314005)
• Project Period (FY): 2011 – 2013
• Keywords: 概日リズム / 生物時計 / 進化 / 多様性 / 光 / 温度

Research Abstract

Circadian clocks are intrinsic, entrainable mechanism that generate biological rhythms with approximately a 24 h period. Circadian rhythms are widespread in many biological processes of almost all organisms including plants. A clock provides plants with an adaptive advantage to anticipate and respond to daily changes in environmental conditions such as day and night, and seasonal changes in photoperiod. Oscillator components to define the molecular mechanism of circadian clock in plants are composed of plant specific proteins. In this study, biochemical properties of central oscillator proteins, pseudo-response regulator (PRR) family were investigated. It was revealed that PRRs function as transcriptional repressors in the central oscillator circuit. In vitro phosphotransfer experiment made it possible to show that a Bryophyte PRR, which is thought to be a prototype of PRR family, has phospho-accepter activity, suggesting that PRRs were diversified from a bona fide response regulator.

Research Products

• [Journal Article] Transcription of ST2A encoding a sulfotransferase family protein that is involved in jasmonic acid metabolism is controlled according to the circadian clock- and PIF4/PIF5-mediated external coincidence mechanism in Arabidopsis thaliana (2013)
  • Author(s): Yamashino, T., Kitayama, M., Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 2454-2460
• [Journal Article] Clock-Controlled and FLOWERING LOCUS T (FT)-Dependent Photoperiodic Pathway in Lotus japonicus II : Characterization of a MicroRNA Implicated in the Control of Flowering Time (2013)
  • Author(s): Yamashino, T., Yamawaki, S., Hagui, E., Ishida, K., Ueoka-Nakanishi, H., Nakamichi, N. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 1179-1185
• [Journal Article] Clock-controlled and FLOWERING LOCUS T (FT)-dependent photoperiodic pathway in Lotus japonicus I : verification of the flowering-associated function of an FT homolog (2013)
  • Author(s): Yamashino, T., Yamawaki, S., Hagui, E., Ueoka-Nakanishi, H., Nakamichi, N., Ito, S. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 747-753
• [Journal Article] Verification at the protein level of the PIF4-mediated external coincidence model for the temperature-adaptive photoperiodic control of plant growth in Arabidopsis thaliana (2013)
  • Author(s): Yamashino, T., Nomoto, Y., Lorrain, S., Miyachi, M., Ito, S., Nakamichi, N., Fankhauser, C. and Mizuno, T.
  • Journal Title: Plant Signal Behav Volume: 8
• [Journal Article] From a repressilator-based circadian clock mechanism to an external coincidence model responsible for photoperiod and temperature control of plant architecture in Arabodopsis thaliana (2013)
  • Author(s): Yamashino, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 10-16
• [Journal Article] Molecular mechanisms of circadian rhythm in Lotus japonicus and Arabidopsis thaliana are sufficiently compatible to regulate heterologous core clock genes robustly (2012)
  • Author(s): Ueoka-Nakanishi, H., Yamashino, T., Ishida, K., Kamioka, M., Nakamichi, N. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 2332-2334
• [Journal Article] Circadian clock and PIF4-mediated external coincidence mechanism coordinately integrates both of the cues from seasonal changes in photoperiod and temperature to regulate plant growth in Arabidopsis thaliana (2012)
  • Author(s): Nomoto, Y., Kubozono, S., Miyachi, M., Yamashino, T., Nakamichi, N. and Mizuno, T.
  • Journal Title: Plant Signal Behav Volume: 8
• [Journal Article] A circadian clock- and PIF4-mediated double coincidence mechanism is implicated in the thermosensitive photoperiodic control of plant architectures in Arabidopsis thaliana (2012)
  • Author(s): Nomoto, Y., Kubozono, S., Miyachi, M., Yamashino, T., Nakamichi, N. and Mizuno, T.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 1965-1973
• [Journal Article] Circadian clock- and PIF4-controlled plant growth : a coincidence mechanism directly integrates a hormone signaling network into the photoperiodic control of plant architectures in Arabidopsis thaliana (2012)
  • Author(s): Nomoto, Y., Kubozono, S., Yamashino, T., Nakamichi, N. and Mizuno, T.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 1950-1964
• [Journal Article] Transcriptional repressor PRR5 directly regulates clock-output pathways (2012)
  • Author(s): Nakamichi, N., Kiba, T., Kamioka, M., Suzuki, T., Yamashino, T., Higashiyama, T., Sakakibara, H. and Mizuno, T.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109 Pages: 17123-17128
• [Journal Article] Characterization of shade avoidance responses in Lotus japonicus (2011)
  • Author(s): Ueoka-Nakanishi, H., Hori, N., Ishida, K., Ono, N., Yamashino, T., Nakamichi, N. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 2148-2154
• [Journal Article] Light-responsive double B-box containing transcription factors are conserved in Physcomitrella patens (2011)
  • Author(s): Yamawaki, S., Yamashino, T., Nakamichi, N., Nakanishi, H. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 2037-2041
• [Journal Article] Functional characterization of HY5 homolog genes involved in early light-signaling in Physcomitrella patens (2011)
  • Author(s): Yamawaki, S., Yamashino, T., Nakanishi, H. and Mizuno, T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 1533-1539
• [Journal Article] Phytochrome-interacting factor 4 and 5 (PIF4 and PIF5) activate the homeobox ATHB2 and auxin-inducible IAA29 genes in the coincidence mechanism underlying photoperiodic control of plant growth of Arabidopsis thaliana (2011)
  • Author(s): Kunihiro, A., Yamashino, T., Nakamichi, N., Niwa, Y., Nakanishi, H. and Mizuno, T.
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 1315-1329
• [Journal Article] Heterologous expression and functional characterization of a Physcomitrella Pseudo response regulator homolog, PpPRR2, in Arabidopsis (2011)
  • Author(s): Satbhai, S. B., Yamashino, T., Mizuno, T. and Aoki, S.
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 786-789
• [Journal Article] Pseudo-response regulator (PRR) homologues of the moss Physcomitrella patens : insights into the evolution of the PRR family in land plants (2011)
  • Author(s): Satbhai, S. B., Yamashino, T., Okada, R., Nomoto, Y., Mizuno, T., Tezuka, Y., Itoh, T., Tomita, M., Otsuki, S. and Aoki, S.
  • Journal Title: DNA Res Volume: 18 Pages: 39-52
• [Presentation] Ambient temperature signal feeds into the circadian clock transcriptional circuitry through the EC nighttime repressor in Arabidopsis thaliana (2014)
  • Author(s): 山篠貴史,野本友司,水野猛
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
  • Year and Date: 20140318-20
• [Presentation] Temperature signal feeds into the circadian clock through the evening complex to regulate PIF4-dependent temperature-adaptive control of hypocotyl elongation in Arabidopsis thaliana (2014)
  • Author(s): 野本友司,山篠貴史,水野猛
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
  • Year and Date: 20140318-20
• [Presentation] 高等植物の時計関連遺伝子PRR5の発現制御機構の解析 (2014)
  • Author(s): 神岡真理,光田展隆,大宮あけみ,山篠貴史,高木優,水野猛,中道範人
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
  • Year and Date: 20140318-20
• [Presentation] 概日時間情報はPRRタンパク質を介して出力現象に伝達する (2013)
  • Author(s): 中道範人、木羽隆敏、神岡真理、鈴木孝征、山篠貴史、東山哲也、榊原均、水野猛
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] 日長と気温変化に応答した胚軸や葉身の伸長制御機構 (2013)
  • Author(s): 野本友司、宮地美希、山篠貴史、中道範人、水野猛
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] ゲノム情報を利用したミヤコグサの花成制御機構に関する検証 (2013)
  • Author(s): 山篠貴史,山脇沙織,羽喰英美,中西華代,中道範人,水野猛
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] Comprehensive insight into the circadian clock of Lotus japonicas (2013)
  • Author(s): Kai Ishida, Hanayo Nakanishi, Takafumi Yamashino, Mari Kamioka, Norihito Nakamichi, Takeshi Mizuno
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] 概日時計に関連したPRR5遺伝子の発現を制御する新規転写因子 (2013)
  • Author(s): 神岡真理、光田展隆、山溝千尋、大宮あけみ、山篠貴史、高木優、水野猛、中道範人
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130300
• [Presentation] Currnt view as to the molecular mechanisms of plant circadian clock with special emphasis on the pseudo-response regulators, PRRs, of Arabidopsis thaliana (2012)
  • Author(s): Kai Ishida, Takafumi Yamashino, Takeshi Mizuno
  • Organizer: The 13th International EMBL PhD Symposium
  • Place of Presentation: Heidelberg
  • Year and Date: 20121100
• [Presentation] シロイヌナズナの概日時計と光周性成長制御(I):外的符合モデルとホメオボックス転写因子ATHB2 (2012)
  • Author(s): 野本友司,国広篤史,中西華代,中道範人,山篠貴史,水野猛
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] シロイヌナズナの概日時計と光周性成長制御(II):外的符合モデルとホルモン応答ネットワーク (2012)
  • Author(s): 久保園沙織,野本友司,神岡真理,中西華代,中道範人,山篠貴史,水野猛
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] ミヤコグサにおける二成分制御系と根粒形成機構 (2012)
  • Author(s): 石田快,山篠貴史,水野猛
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] ヒネツリガネゴケにおける進化的に保存された光応答転写因子群の解析 (2012)
  • Author(s): 山脇沙織,石田快,山篠貴史,水野猛
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] ミヤコグサにおける避陰反応と分枝制御 (2012)
  • Author(s): 中西華代,堀菜七子,山篠貴史,水野猛
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] Involvement of circadian clock in photoperiodic control of plant growth of Arabidopsis (2012)
  • Author(s): Takafumi Yamashino, Takeshi Mizuno
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~microbio/