2013 Fiscal Year Final Research Report
The search for novel biomarkers by comprehensive identification of human N-myristoylated proteins
| Project/Area Number |
23580136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
UTSUMI TOSHIHIKO 山口大学, 医学(系)研究科(研究院), 教授 (20168727)
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| Project Period (FY) |
2011 – 2013
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| Keywords | N-ミリストイル化 / バイオマーカー探索 / タンパク質脂質修飾 / 細胞情報伝達 / タンパク質翻訳後修飾 |
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| Research Abstract |
In order to search for novel human N-myristoylated proteins that can be applied for biomarkers, the susceptibility of human cDNA clones in human cDNA resources to protein N-myristoylation was evaluated by metabolic labeling of proteins expressed using an insect cell-free protein synthesis system. For this analysis, 178 cDNA clones with an N-terminal Met-Gly motif were selected as potential candidates from ~6,300 Kazusa ORFeome project (KOP) human cDNA clones. The susceptibility of these cDNA clones to protein N-myristoylation was evaluated by metabolic labeling experiments both in an insect cell-free protein synthesis system and in transfected mammalian cells. As a result, the products of 35 out of ~6,300 cDNA clones were found to be novel human N-myristoylated proteins. These novel N-myristoylated proteins contain not only physiologically important proteins but also many disease-associated proteins that can be applied for biomarkers.
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[Journal Article] Protein N-myristoylation is required for cellular morphological changes induced by two formin family proteins, FMNL2 and FMNL32012
Author(s)
Moriya K, Yamamoto T, Takamitsu E, Matsunaga Y, Kimoto M, Fukushige D, Kimoto C, Suzuki T, Utsumi T.
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Journal Title
Bioscience, Biotechnology and Biochemistry
Volume: 76
Pages: 1201-1209
Peer Reviewed
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[Journal Article] Serum-dependent export of protoporphyrin IX by ATP-binding cassette transporter G2 in T24 cells2011
Author(s)
Ogino T, Kobuchi H, Munemoto K, Fujita H, Yamamoto M, Utsumi T, Inoue K, Shuin T, Sasaki J, Inoue M, Utsumi K.
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Journal Title
Molecular and Cellular Biochemistry
Volume: 358
Pages: 297-307
Peer Reviewed
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