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2013 Fiscal Year Final Research Report

Functions of sulfotransferases and their signal transductions

Research Project

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Project/Area Number 23580138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionUniversity of Miyazaki

Principal Investigator

SUIKO Masahito  宮崎大学, 農学部, 教授 (00128357)

Co-Investigator(Kenkyū-buntansha) SAKAKIBARA Yoichi  宮崎大学, 大学院農学工学総合研究科, 教授 (90295197)
Project Period (FY) 2011 – 2013
Keywords酵素 / 硫酸転移酵素 / 薬物代謝 / 硫酸化 / プロスタグランジン / オーファン / ゼブラフィッシュ
Research Abstract

In this research project, we focused on the evaluation of physiological relevance of two kinds of orphan sulfotransferases (SULTs) such as SULT4A1 and SULT7A1 whose biochemical/physiological functions are unknown. RT-PCR and in situ hybridization analyses using zebrafish embryos revealed that SULT4A1 was specifically expressed in whole brain and spinal cord. These results indicate that SULT4A1 plays important roles in the early development of a nervous system of zebrafish embryos. On the other hand, detail characterization of mouse SULT7A1 demonstrated that SULT7A1 exhibited the specific sulfating activity toward cyclopentenone prostaglandins including PGA2 and 15-deoxy-delta-12,14-PGJ2 (15d-PGJ2), which were endogenous metabolites of PGE2 and PGD2, respectively, but not PGE2 and PGD2.
These studies would encourage us to investigate additional biochemical characterization of these orphan SULTs in the pursuit of yet-to-be-defined function.

  • Research Products

    (26 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana2014

    • Author(s)
      Hashiguchi, T., Sakakibara, Y., Kurogi, K., Yamasaki, M., Nishiyama, K., Akashi, R., Liu, M.-C. and Suiko, M.
    • Journal Title

      J . Biochem

      Volume: 155(2) Pages: 91-97

    • DOI

      10.1093/jb/mvt102

    • Peer Reviewed
  • [Journal Article] The use of zebrafish as a model system for investigating the role of the SULTs in the metabolism of endogenous compounds an xenobiotics2013

    • Author(s)
      Kurogi, K., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      Drug Metab Rev

      Volume: 45(4) Pages: 431-440

    • DOI

      10.3109/03602532.2013.835629

    • Peer Reviewed
  • [Journal Article] Identification and characterization of a novel kaempferol sulfotransferase from Arabidopsis thaliana2013

    • Author(s)
      Hashiguchi, T., Sakakibara, Y., Hara, Y., Kurogi, K., Akashi, R., Liu, M.-C. and Suiko, M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 434(4) Pages: 829-835

    • DOI

      10.1016/j.bbrc.2013.04.022

    • Peer Reviewed
  • [Journal Article] Crystal structure of human tyrosylprotein sulfotransferase-2: Insights into the mechanism of protein tyrosine sulfation reaction2013

    • Author(s)
      Teramoto, T., Fujikawa, Y., Kawaguchi, Y., Kurogi, K., Soejima, M., Adachi, R., Nakanishi, Y., Mishiro-Sato, M., Liu, M.-C., Sakakibara, Y., Suiko, M., Kimura, M., and Kakuta, Y.
    • Journal Title

      Nature Commun

      Volume: 4 Pages: 1572

    • DOI

      10.1038/ncomms2593

    • Peer Reviewed
  • [Journal Article] Ethanol sulfation by the human cytosolic sulfotransferases : a systematic analysis2012

    • Author(s)
      Kurogi, K., Davidson, G., Mohammed, Y.I., Williams, F.E., Liu, M.Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      Bio. Pharm. Bull

      Volume: 35(12) Pages: 2180-2185

    • Peer Reviewed
  • [Journal Article] Sulfation of buprenorphine, pentazaocine, and naloxone by human cytosolic sulfotransferases2012

    • Author(s)
      Kurogi, K., Chen, M., Lee, Y., Shi, B., Yang, T., Liu, M.Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      Drug Metab. Lett

      Volume: 6(2) Pages: 109-115

    • Peer Reviewed
  • [Journal Article] Concerted actions of the catechol O-methyltransferase and the cytosolic sulfotransferase SULT1A3 in the metabolism of catecholic drugs2012

    • Author(s)
      Kurogi, K., Alazizi, A., Liu, M.Y., Sakakibara, Y., Suiko, M., Sugahara, T. and Liu, M.-C.
    • Journal Title

      Biochem. Pharmacol

      Volume: 84 Pages: 1186-1195

    • DOI

      10.1016/j.bcp.2012.08.009

    • Peer Reviewed
  • [Journal Article] Sulfation of ractopamine and salbutamol by the human cytosolic sulfotranasferases2012

    • Author(s)
      Ko, K., Kurogi, K., Davidson, G., Liu, M.-Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      J . Biochem

      Volume: 152(3) Pages: 275-283

    • DOI

      10.1093/jb/mvs073

    • Peer Reviewed
  • [Journal Article] Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST52012

    • Author(s)
      Mohammed, Y.I., Kurogi, K., Shaban, A.A., Xu, Z., Liu, M.Y., Williams, F.E., Sakakibara, Y., Suiko, M., Bhuiyan, S. and Liu, M.-C.
    • Journal Title

      Aquat. Toxicol

      Volume: 112-113 Pages: 11-18

    • DOI

      10.1016/j.aquatox.2012.01.015

    • Peer Reviewed
  • [Journal Article] タンパク質の翻訳後修飾としてのチロシン硫酸化2012

    • Author(s)
      水光正仁、榊原陽一
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 70(3) Pages: 201-205

  • [Journal Article] Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases2011

    • Author(s)
      Hashiguchi, T., Kurogi, K., Sakakibara, Y., Yamasaki, M., Nishiyama, K., Yasuda, S., Liu, M.-C. and Su iko, M.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 1951-1956

    • Peer Reviewed
  • [Journal Article] A comparative study of the sulfation of bile acids and a bile alohol by the Zebra danio (Danio rerio) and human cytosolic sulfotransferases (SULTs)2011

    • Author(s)
      Kurogi, K., Krasowski, M.D., Injeti, E., Liu, M.-Y., Williams, F.E., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      J . Steroid Biochem. Mol. Biol

      Volume: 127(3-5) Pages: 307-14

    • DOI

      10.1016/j.jsbmb.2011.07.011

    • Peer Reviewed
  • [Journal Article] Identification, characterization, and ontogenic study of a catechol O- methyltransferase from zebrafish2011

    • Author(s)
      Alazizi, A., Kurogi, K., Sakakibara, Y., Suiko, M. and Liu, M.-C.
    • Journal Title

      Aquat. Toxicol

      Volume: 102 Pages: 18-23

    • DOI

      10.1016/j.aquatox.2010.12.016

    • Peer Reviewed
  • [Journal Article] Sulfation of chlorotyrosine and nitrotyrosine by human lung endothelial and epithelial cells : Role of the human SULT1A32011

    • Author(s)
      Yasuda, S., Yasuda, T., Liu, M.-Y., Shetty, S., Idell, S., Boggaram, V., Suiko, M., Sakakibara, Y. and Liu, M.-C.
    • Journal Title

      Toxicol. Appl. Pharmacol

      Volume: 251(2) Pages: 104-109

    • DOI

      10.1016/j.taap.2010.12.006

    • Peer Reviewed
  • [Journal Article] 薬物代謝と農薬の効能:特に硫酸化について2011

    • Author(s)
      橋口拓勇、榊原陽一、水光正仁
    • Journal Title

      日本農薬学会誌

      Volume: 36(2) Pages: 297-299

  • [Presentation] ゼブラフィッシュグルタチオン転移酵素の同定とその諸性質検討2014

    • Author(s)
      黒木勝久、榊原陽一、Liu Ming-Cheh、水光正仁
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Year and Date
      20140300
  • [Presentation] タンパク質蛍光標識技術を用いたS-ニトロシル化タンパク質の網羅的解析2014

    • Author(s)
      芳村俊広、黒木勝久、水光正仁、榊原陽一
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Year and Date
      20140300
  • [Presentation] 代謝工学的手法による食品機能性成分硫酸体の産生技術開発2013

    • Author(s)
      下平武彦、黒木勝久、橋口拓勇、Ming-Cheh Liu、榊原陽一、水光正仁
    • Organizer
      第20回日本生物工学会九州支部佐賀大会
    • Year and Date
      20131200
  • [Presentation] ヒトタンパク質チロシン硫酸転移酵素の構造機能解析2013

    • Author(s)
      西依利晃、古城英貴、大坪怜央、川口喜郎、黒木勝久、榊原陽一、水光正仁、木村誠、角田佳充
    • Organizer
      第37回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      雲仙
    • Year and Date
      20130900
  • [Presentation] プロテオーム解析によるフラボノイド硫酸体の生理機能解明2013

    • Author(s)
      橋口拓勇、芳村俊宏、榊原陽一、下平武彦、黒木勝久、水光正仁
    • Organizer
      第37回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      雲仙
    • Year and Date
      20130900
  • [Presentation] Tyrosylprotein Sulfotransferase活性阻害が期待される食品成分の探索2013

    • Author(s)
      福島文恵、榊原陽一、黒木勝久、角田佳充、木村誠、Ming-Cheh Liu、水光正仁
    • Organizer
      第37回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      雲仙
    • Year and Date
      20130900
  • [Presentation] Two-dimensional gel electrophoresis analysis of fravonoid sulfate effects on A. thaliana T87 cells2013

    • Author(s)
      Hashiguchi, T., Sakakibara, Y., Shimohira, T., Yoshimura, T., Kurogi, K., Hara, Y., Liu, M.-C., Suiko, M.
    • Organizer
      The 12th Human Proteome Organisation World Congress (HUPO 2013)
    • Place of Presentation
      Yokohama
    • Year and Date
      20130900
  • [Presentation] Proteomic analysis of the cellular signal regulation mechanism by cholesterol sulfation2013

    • Author(s)
      Yoshimura, T., Kurogi, K., Liu, M.-C., Suiko, M., Sakakibara, Y.
    • Organizer
      The 12th Human Proteome Organisation World Congress (HUPO 2013)
    • Place of Presentation
      Yokohama
    • Year and Date
      20130900
  • [Presentation] 硫黄の生体利用に関する最新知見と新展開:硫酸転移酵素の多様な機能2013

    • Author(s)
      榊原陽一、黒木勝久、角田佳充、Ming-Cheh Liu、水光正仁
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20130900
  • [Presentation] シロイヌナズナ硫酸転移酵素によるフラボノイド硫酸化2013

    • Author(s)
      原洋介、橋口拓勇、下平武彦、黒木勝久、榊原陽一、Liu Ming-Cheh、水光正仁
    • Organizer
      日本農芸化学会関西・中四国・西日本支部2013年度合同広島大会
    • Place of Presentation
      広島
    • Year and Date
      20130900
  • [Remarks] ホームページ

    • URL

      http://tpstsult.web.fc2.com/

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Published: 2015-07-16  

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