Study on a factor in silkglands that suppresses defense responses of plants during infestation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yamaguchi University
• Principal Investigator: MATSUI Kenji 山口大学, 医学(系)研究科(研究院), 教授 (90199729)
• Co-Investigator(Kenkyū-buntansha): OZAWA Rika 京都大学, 生態学研究センター, 研究員 (90597725) ; KOBAYASHI Jun 山口大学, 農学部, 教授 (70242930)
• Project Period (FY): 2011 – 2013
• Keywords: みどりの香り / カイコ / 絹糸腺 / リポキシゲナーゼ / 植物昆虫相互作用

Research Abstract

We found an activity to suppress formation of green leaf volatiles in the silk gland of silkworms. After purification, we obtained a protein of 31 kDa, that showed high fatty acid hydroperoxide dehydratase activity. When various isomers of substrates were used, the enzyme showed very strict substrate specificity to 13S-ZE isomer. In fact, this isomer is the one accountable to formation of green leaf volatiles in plants. From these observation, it was suggested that the enzyme is not only the antioxidative enzyme but probably involved in oxylipin metabolism either in plants or in the insects. When we analyzed the gene encoding the enzyme, we found it was a gene that had not been assigned. We are now trying to get a transgenic silkworm that has deletion of the gene, thereafter, we will be able to see the physiological and ecological significances of the enzyme activity for silkworms.

Research Products

• [Journal Article] Formation of 1-octen-3-ol from Aspergillus flavus conidia is accelerated after disruption of cells independently of Ppo oxygenases, and is not a main cause of inhibition of germination (2014)
  • Author(s): Kana Miyamoto, Tomoko Murakami, Pattana Kakumyan, Nancy P. Keller, Kenji Matsui
  • Journal Title: PeerJ
  • DOI: 10.7717/peerj.395
  • Peer Reviewed
• [Journal Article] Intake and transformation to a glycoside of (Z)-3-hexenol from infested neighbors reveals a mode of plant odor reception and defense (2014)
  • Author(s): Koichi Sugimoto, Kenji Matsui, Yoko Iijima, Yoshihiko Akakabe, Shoko Muramoto, Rika Ozawa, Masayoshi Uefune, Ryosuke Sasaki, Kabir Md. Alamgir, Shota, Akitake, Tatsunori Nobuke, Ivan Galis, Koh Aoki, Daisuke Shibata, Junji Takabayashi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Pages: 7144-7149
  • Peer Reviewed
• [Journal Article] Traumatin- and dinortraumatin-containing galactolpids in Arabidopsis. Their formation in tissue-disrupted leaves as counterparts of green leaf volatiles (2013)
  • Author(s): Anna Nakashima, Stephan H. von Reuss, Hiroyuki Tasaka, Misaki Nomura, Satoshi Mochizuki, Yoko Iijima, Koh Aoki, Daisuke Shibata, Wilhelm Boland, Junji Takabayashi, Kenji Matsui
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 26078-26088
  • Peer Reviewed
• [Presentation] Purification and characterization of a factor suppressing green leaf volatile formation in silk gland of silkworms (2013)
  • Author(s): Hiroki Takai, Saki Fujii, Rika Ozawa, Hideo Dohra, Tosshiyuki Ohnishi, Jun Kobayashi, Junji Takabayashi, Kenji Matsui
  • Organizer: 5^<th> Asian Symposium on Plant Lipids
  • Place of Presentation: Gwangju, Korea
  • Year and Date: 2013-11-29
• [Presentation] カイコ絹糸腺のみどりの香り精製抑制因子の精製とその性質に着いて (2013)
  • Author(s): 高井嘉樹、藤井沙季、小澤理香、道羅英夫、大西利幸、小林淳、高林純示、松井健二
  • Organizer: 日本農芸化学会支部合同広島大会
  • Place of Presentation: 県立広島大学広島キャンパス,広島市
  • Year and Date: 2013-09-05
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/~matsui/