2013 Fiscal Year Final Research Report
Study on the olfactory imprinting-related brain regions in anadromous salmon: Neuroanatomical analysis for the primary olfactory projected regions in salmon brain.
| Project/Area Number |
23580241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUDO Hideaki 北海道大学, 水産科学研究科(研究院), 准教授 (40289575)
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| Project Period (FY) |
2011 – 2013
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| Keywords | サケ / 母川回帰 / 嗅神経系 / 母川刷込 / 嗅球 |
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| Research Abstract |
In order to clarify the imprinting-related brain regions in anadromous salmon (Oncorhynchus spp.), we visualized the primary olfactory projected regions in salmon brain by neuroanatomical techniques using DiI fluorescent tracer. In order to distinguish the type of the olfactory receptor cells which constitutes each primary olfactory projection, we analyzed immunohistochemically as olfactory receptor cell markers taking notice of the olfactory marker protein (OMP) and glutathione S-transferase pi (GST). This method was able to distinguish ciliated and microvillus olfactory receptor cells. In addition, the localization of G protein alpha subunit olf (Golf) in regard of a olfaction-related second messenger cascade was immunohistochemically analyzed using the confocal laser scanning microscope, and had expression of Golf identified for the first time in the salmon olfactory system.
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