Functional profiles of skin mucus in bivalve mollusks involved in a surface barrier against bacterial infection

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580245
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Keisuke 東北大学, (連合)農学研究科(研究院), 准教授 (80240662)
• Project Period (FY): 2011 – 2013
• Keywords: 生体防御 / 二枚貝 / マガキ / 体表面粘液 / 抗菌活性 / リゾチーム / キチナーゼ

Research Abstract

Shellfish belongs to the phylum Mollusca and is mainly comprised of bivalves and gastropods. The Phylum Mollusca is one of the largest and numerous groups in the animal kingdom. Shellfish and microorganisms coexist in the biosphere in numerous ways. Thus, bivalves have evolved sensitive mechanisms for recognizing pathogens and an array of strategies to defend themselves against attacks by microorganisms such as bacteria, fungi, and parasites. An oft-asked question is how invertebrates including shellfish survive against pathogenic microorganisms without an adaptive immune system. Indeed, invertebrates do not have lymphocytes and do not produce antibodies. Thus, we have investigated that skin mucus from mantle and gill functions as a surface barrier against bacterial infection.

Research Products

• [Journal Article] Involvement of Pacific oyster CgPGRP-S1S in bacterial recognition, agglutination and granulocyte degranulation (2014)
  • Author(s): Iizuka, M., Nagasaki, T., Takahashi, KG., Osada, M.and Itoh, N.
  • Journal Title: Dev. Comp. Immunol Volume: 43 Pages: 30-34
  • DOI: 10.1016/j.dci.2013.10.011
  • Peer Reviewed
• [Journal Article] Shellfish Bio-Defense, volume 5.5.35 Fish Disease (2014)
  • Author(s): Takahashi, KG.
  • Journal Title: UNESCO EOLSS Encyclopedia Pages: 11-16
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas (2013)
  • Author(s): Okada, Y., Yamaura, K., Suzuki, T., Itoh, N., Osada, M.and Takahashi, KG.
  • Journal Title: Comp. Biochem. Physiol Volume: 165 Pages: 83-89
  • DOI: 10.1016/j.cbpb.2013.03.008
  • Peer Reviewed
• [Presentation] Assessment of unique phagocytic properties of the Pacific oyster hemocytes against yeast and yeast cell wall derivatives (2013)
  • Author(s): Takahashi, KG.
  • Organizer: The 5^<th> International Oyster Symposium
  • Place of Presentation: Saigon Exhibition Conference Center, Ho Chi Minh, Vietnam
  • Year and Date: 2013-12-12
  • Invited
• [Presentation] Formation of spheroidal hemocyte aggregates of Crassostrea gigas hemocytes involved in protein kinase C signaling pathways in bio-defense mechanisms (2013)
  • Author(s): Takahashi, KG.
  • Organizer: The 5^<th> International Oyster Symposium
  • Place of Presentation: Saigon Exhibition Conference Center, Ho Chi Minh, Vietnam
  • Year and Date: 2013-12-12
• [Presentation] 簡易測定キットにより調べた海産二枚貝の血リンパの酵素活性の特徴 (2013)
  • Author(s): 高橋計介、伊藤直樹、尾定誠
  • Organizer: 日本水産増殖学会第12回大会
  • Place of Presentation: 鹿児島大学、鹿児島市
  • Year and Date: 2013-10-14
• [Presentation] 二枚貝の生体防御における活性酸素の機能 (2013)
  • Author(s): 高橋計介
  • Organizer: 第24回日本生体防御学会・シンポジウムI
  • Place of Presentation: くまもと森都心プラザ、熊本市
  • Year and Date: 2013-07-10
  • Invited
• [Presentation] マガキ血球の2つの細胞亜集団、顆粒球および無顆粒球の遺伝子発現解析、JST・研究成果展開事業(先端計測分析技術・機器開発プログラム) (2013)
  • Author(s): 高橋計介
  • Organizer: 平成25年度サイトビジット検討会
  • Place of Presentation: 放射線医学総合研究所、千葉市
  • Year and Date: 2013-05-29
• [Presentation] 海産二枚貝アカガイに対するγ線の照射効果 (2013)
  • Author(s): 高橋計介・中島菜花子・藤森亮
  • Organizer: 平成25年度HIMAC共同利用研究成果発表会
  • Place of Presentation: ホテルポートプラザちば、千葉市
  • Year and Date: 2013-04-22
• [Presentation] defense mechanisms in bivalve molluscs, Memorial symposium on discovery of intermediate snail hosts of Schistosoma japonicum (2013)
  • Author(s): Takahashi, KG.
  • Organizer: The 82^<nd> Annual Meeting of JSP
  • Place of Presentation: Tokyo Medical and Dental University, Tokyo
  • Year and Date: 2013-03-30
  • Invited
• [Presentation] A Peptidoglycan Recognition Protein Induces Hemocyte Degranulation in the Pacific Oyster, Crassostrea gigas (2013)
  • Author(s): Iizuka, M, Nagasaki, T., Takahashi, KG., Osada, M., and Itoh, N.
  • Organizer: Aquaculture 2013, World Aquaculture Society
  • Place of Presentation: Nashville, Tennessee, USA
  • Year and Date: 2013-02-25
• [Presentation] 海産二枚貝サルボウ組織に対する重粒子線照射の影響 (2012)
  • Author(s): 高橋計介・中島菜花子・藤森亮・伊藤直樹・尾定誠
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 水産大学校、山口県下関市
  • Year and Date: 2012-09-15
• [Presentation] マガキ血球における貪食胞内酸性化 (2012)
  • Author(s): 阿部史隆・福田陽一・高橋計介・伊藤直樹・尾定誠
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 水産大学校、山口県下関市
  • Year and Date: 2012-09-15
• [Presentation] 海産二枚貝アカガイの造血組織の対する重粒子線照射の効果 (2012)
  • Author(s): 高橋計介・中島菜花子・藤森亮
  • Organizer: HIMAC共同利用研究成果発表会
  • Place of Presentation: ホテルポートプラザちば、千葉市
  • Year and Date: 2012-04-23
• [Presentation] マガキPGRP-S1Sによるグラム陰性菌認識 (2011)
  • Author(s): 飯塚真生・長崎稔拓・高橋計介・尾定誠・伊藤直樹
  • Organizer: 日本比較免疫学会第23回学術集会
  • Place of Presentation: 海洋研究開発機構、神奈川県横須賀市
  • Year and Date: 2011-08-22
• [Book] Bondad-Reantaso, MG., Jones, JB., Corsin, F., Aoki, T. (eds), Diseases in Asian Aquaculture VII, Fish Health Section, Asian Fisheries Society, Selangor (2011)
  • Author(s): Takahashi, KG. and Itoh, N.
  • Total Pages: 385(93-102)
  • Publisher: Lysozymes in molluscs