2013 Fiscal Year Final Research Report
Investigation of resistance mechanism of ayu against cold water disease by comprehensive analysis of antimicrobial peptides
| Project/Area Number |
23580254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
IIJIMA NORIAKI 広島大学, 生物圏科学研究科, 教授 (90136143)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSAWA Shuichi 広島大学, 大学院生物圏科学研究科, 教 授 (80130037)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 魚病 / 生体防御 / 抗菌ペプチド |
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| Research Abstract |
We report here the isolation of two novel antimicrobial peptides from the spleen of Ayu, Plecoglossus altivelis. Antimicrobial peptides were purified from the acid extract of Ayu spleen with ion-exchange chromatography, gel-filtrarion chromatography, and two-step reversed-phase HPLC. Peptide sequences were determined by a combination of Edman degradation and ESI mass spectrometry. Two antimicrobial peptides, named plecoglosin-1 and -2, consist of 11 and 10 amino acids, respectively. Synthetic plecoglosin-1 and -2 displayed broad-spectrum bactericidal activity against Gram-negative and Gram-positive bacteria including Escherichia coli, Bacillus subtilis, and fish and crustacean pathogens.
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