2013 Fiscal Year Final Research Report
Identification of hematopoietic stem cells and hematopoietic organ in dolphin using CD34
| Project/Area Number |
23580267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Nihon University |
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Principal Investigator |
ITOU Takuya 日本大学, 生物資源科学部, 准教授 (20307820)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Miwa 日本大学, 生物資源科学部, 准教授 (70409069)
KOIE Hiroshi 日本大学, 生物資源科学部, 准教授 (20267040)
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| Project Period (FY) |
2011 – 2013
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| Keywords | イルカ / CD34 / 分離 / 造血幹細胞 |
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| Research Abstract |
Identification of the hematopoietic organ and isolation method of the hematopoietic stem cells were examined in dolphin. To isolate hematopoietic cells, dolphin CD34 gene was identified, and recombinant dolphin CD34 protein and its specific antibody were produced. While the distribution of CD34 in a dolphin was immunohistochemically determined using this antibody, it proved that bone marrow is a hematopoietic organ containing the CD34 positive hematopoietic stem cells which have the differentiation potency to the blood cell. Separation and concentration method of the hematopoietic stem cells from bone marrow and peripheral blood was established in dolphin.
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