Distribution and function of tetrodotoxin-binding proteins in the pufferfish Takifugu rubripes -elucidation from a viewpoint of sexual maturation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Nagasaki University
• Principal Investigator: ARAKAWA Osamu 長崎大学, 水産・環境科学総合研究科, 教授 (40232037)
• Co-Investigator(Kenkyū-buntansha): TAKATANI Tomohiro 長崎大学, 水産・環境科学総合研究科, 教授 (90304972)
• Project Period (FY): 2011 – 2013
• Keywords: テトロドトキシン / フグ / ツムギハゼ / テトロドトキシン結合性タンパク質（PSTBP） / 成熟

Research Abstract

Various tetrodotoxin (TTX) administration experiments using non-toxic cultured pufferfish, and investigations on internal TTX distribution of several wild pufferfish species and the goby Yongeichthys criniger demonstrated that TTX kinetics inside the body changes depending on the skin/liver development due to growth, and ovary development/maturation due to sexual maturation in these TTX-bearing fish. Moreover, searches for homologous genes of the TTX-binding protein (PSTBP) on several pufferfish species indicated that Takifugu rubripes possesses 3 candidate genes, 2 of which actually exist as proteins in the blood plasma, and that the other 3 toxic species also have 2-domain type candidate genes, and the difference in their sugar chain binding sites may be related to the toxin accumulation ability.

Research Products

• [Journal Article] Change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured pufferfish Takifugu rubripes depending of its development stage (2013)
  • Author(s): R. Tatsuno, M. Shikina, Y. Shirai, J. Wang, K. Soyano, G.N. Nishihara, T. Takatani, O. Arakawa
  • Journal Title: Toxicon Volume: 65 Pages: 76-80
• [Journal Article] Maturation- associated changes in the internal distribution of tetrodotoxin in the female goby Yongeichthys criniger (2013)
  • Author(s): R. Tatsuno, M. Shikina, K. Soyano, K. Ikeda, T. Takatani, O. Arakawa
  • Journal Title: Toxicon Volume: 63 Pages: 64-69
• [Journal Article] RT-PCR- and MALDI-TOF mass spectrometry-based identification and discrimination of isoforms homologous to pufferfish saxitoxin- and tetrodotoxin- binding protein in the plasma of non-toxic cultured pufferfish (Takifugu rubripes) (2013)
  • Author(s): R. Tatsuno, K. Yamaguchi, T. Takatani, O. Arakawa
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 77 Pages: 208-212
• [Journal Article] Transfer profile of orally and intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu porphyreus (2012)
  • Author(s): J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, T. Takatani, O. Arakawa
  • Journal Title: Food Hyg. Saf. Sci Volume: 53 Pages: 33-38
• [Journal Article] Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu niphobles (2011)
  • Author(s): J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, M. Hamasaki, Y. Sakakura, T. Takatani, O. Arakawa
  • Journal Title: Toxicon Volume: 58 Pages: 565-569
• [Presentation] トラフグに筋肉内投与したTTXの動態-肝臓と皮の毒蓄積様式の相違- (2014)
  • Author(s): 辰野竜平,井樋洸太郎,沖田光玄,山中祐二,高谷智裕,荒川修
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 函館
  • Year and Date: 20140327-31
• [Presentation] Growthassociated changes in internal tetrodotoxin distribution and skin structure in three species of pufferish (2013)
  • Author(s): R. Tatsuno, T. Mine, Y. Yamanaka, K. Ikeda, T. Takatani, O. Arakawa
  • Organizer: 9th International Conference on the Marine Biodiversity and Environmental Fisheries Science of the East China Sea
  • Place of Presentation: Keelung, Sep
  • Year and Date: 20130929-1001
• [Presentation] 無毒養殖トラフグにおけるPSTBP相同アイソフォーム遺伝子の発現 (2013)
  • Author(s): 辰野竜平,山口健一,高谷智裕,荒川修
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] 天然フグ3種における体内毒分布の成長依存的変化 (2013)
  • Author(s): 山中祐二,峰智香,辰野竜平,池田光壱,高谷智裕,荒川修
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20130326-30
• [Presentation] Two proteins homologous to pufferfish saxitoxin- and tetrodotoxin- binding protein (PSTBP) found in the plasma of non-toxic cultured specimens of the pufferfish (Takifugu rubripes) (2012)
  • Author(s): R. Tatusno, K. Yamaguchi, T. Takatani, O. Arakawa
  • Organizer: 17th World Congress of the International Society on Toxinology
  • Place of Presentation: Honolulu
  • Year and Date: 20120708-13
• [Presentation] Transfer profiles of intramuscularly administered tetrodotoxin to artificial hybrid specimens of the pufferfish : Takifugu rubripes × Takifugu niphobles and T. niphobles × T. rubripes, Joint (2012)
  • Author(s): Y. Yamanaka, S. Niina, J. Wang, T. Araki, R. Tatsuno, K. Ikeda, M. Hamasaki, Y. Sakakura, T. Takatani, O. Arakawa
  • Organizer: International Symposium on Marine Science and Technology
  • Place of Presentation: Jeju
  • Year and Date: 2012-10-25
• [Presentation] クサフグ×トラフグ人工交雑個体に筋肉内投与したテトロドトキシンの動態 (2012)
  • Author(s): 新名真也,荒木泰一朗,王俊杰,辰野竜平,濱崎将臣,高谷智裕,荒川修
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-29
• [Presentation] トラフグ×マフグ人工交雑個体の消化管および筋肉内に投与したテトロドトキシンの動態 (2012)
  • Author(s): 王俊杰,荒木泰一朗,辰野竜平,新名真也,池田光壱,高谷智裕,荒川修
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-29
• [Presentation] Growth-mediated change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured specimens of the pufferfish Takifugu rubripes (2011)
  • Author(s): R. Tatsuno, M. Shikina, Y. Shirai, T. Takatani, O. Arakawa
  • Organizer: 8th International Workshop on Oceanography & Fisheries Science of the East China Sea
  • Place of Presentation: Naha
  • Year and Date: 2011-11-25
• [Remarks]
  • URL: http://www2.fish.nagasaki-u.ac.jp/FISH/KENKYU/21arakawa/eisei.html