Toxicity and food safety assessment of hybrid pufferfish

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Nagasaki University
• Principal Investigator: TAKATANI Tomohiro 長崎大学, 大学院水産・環境科学総合研究科, 教授 (90304972)
• Co-Investigator(Kenkyū-buntansha): ARAKAWA Osamu 長崎大学, 大学院水産・環境科学総合研究科, 教授 (40232037)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Shigenori 独立行政法人水産総合研究センター, 増養殖研究所, 主任研究員 (60463105) ; MOCHIOKA Noritaka 九州大学, 大学院農学研究院, 准教授 (40212261)
• Project Period (FY): 2011 – 2013
• Keywords: 食品衛生 / フグ毒（TTX） / 交雑フグ

Research Abstract

We examined to clarify the toxicity, toxin accumulation ability, and the inherited characteristics of hybrid pufferfish, as well as the transfer/accumulation/elimination mechanisms of TTX in the pufferfish body. 20 natural hybrid specimens were collected from coastal waters of Japan, and their parent species were identified by analysis of those mitochondrial and nuclear DNA. Moreover, anatomical distributions of toxicity were clarified by LC/MS analysis. Five hybrids species were contained in 20 specimens, and they were identified as "shimatora", "magoma", "matora", "toragoma" and "torama", respectively. When TTX was administrated to artificial hybrid "torama" and "torakusa", transferred first to the liver and then to the skin and ovary. On the other hand, TTX was transferred also to the testis in male "kusatora".

Research Products

• [Journal Article] 天然トラフグ肝臓の毒性分布 (2013)
  • Author(s): 谷口香織,高尾秀樹,新名真也,山中祐二,岡田行長,中島梨花,王俊杰,辰野竜平,阪倉良孝,高谷智裕,荒川修,野口玉雄
  • Journal Title: 食品衛生学雑誌 Volume: 54 Pages: 128-133
  • Peer Reviewed
• [Journal Article] Difference in the localization of tetrodotoxin between the female and male pufferfish Takifugu niphobles, during spawning (2012)
  • Author(s): S. Itoi, S. Yoshikawa, R. Tatsuno, M. Suzuki, K. Asahina, S. Yamamoto, S. Takanashi, T. Takatani, O. Arakawa, Y. Sakakura, H. Sugita
  • Journal Title: Toxicon Volume: 60 Pages: 1000-1004
  • Peer Reviewed
• [Journal Article] Tetrodotoxin Poisoning Due to Smooth-backed Blowfish Lagocephalus inermis and Toxicity of L. inermis Caught off the Kyushu Coast, Japan (2012)
  • Author(s): Y. Nagashima, T. Matsumoto, K. Kadoyama, S. Ishizaki, S. Taniyama, T. Takatani, O. Arakawa, M. Terayama
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 53 Pages: 85-90
  • Peer Reviewed
• [Journal Article] Transfer Profile of Orally and Intramuscularly Administered Tetrodotoxin to Artificial Hybrid Specimens of the Pufferfish Takifugu rubripes and Takifugu porphyreus (2012)
  • Author(s): J-J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, T. Takatani, O. Arakawa
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 53 Pages: 33-38
  • Peer Reviewed
• [Journal Article] Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu niphobles (2011)
  • Author(s): J-J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, M. Hamasaki, Y. Sakakura, T. Takatani, O. Arakawa
  • Journal Title: Toxicon Volume: 58 Pages: 565-569
  • Peer Reviewed
• [Presentation] 天然トラフグ肝臓の毒性分布 (2013)
  • Author(s): 谷口香織,高尾秀樹,新名真也,山中祐二,岡田行長,中島梨花,王俊杰,辰野竜平,高谷智裕,荒川修,野口玉雄
  • Organizer: 日本食品衛生学会
  • Place of Presentation: 沖縄コンベンションセンター,沖縄
  • Year and Date: 20131121-22
• [Presentation] テトロドトキシンを給餌した腐肉食性小型巻貝2種の毒性 (2013)
  • Author(s): 辰野竜平,反町太樹,谷山茂人,大城直雅,久保弘文,高谷智裕,荒川修
  • Organizer: 日本食品衛生学会
  • Place of Presentation: 沖縄コンベンションセンター,沖縄
  • Year and Date: 20131121-22
• [Presentation] 天然ヒガンフグの麻痺性貝毒蓄積能 (2013)
  • Author(s): 山中祐二,新名真也,山下洋平,辰野竜平,高谷智裕,荒川修
  • Organizer: 日本食品衛生学会
  • Place of Presentation: 沖縄コンベンションセンター,沖縄
  • Year and Date: 20131121-22
• [Presentation] クサフグ×トラフグ人工交雑個体に筋肉内投与したテトロドトキシンの動態 (2012)
  • Author(s): 新名真也,荒木泰一朗,王俊杰,辰野竜平,濱崎将臣,山中祐二,高谷智裕,荒川修
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20120326-30
• [Presentation] トラフグ×マフグ人工交雑個体の消化管および筋肉内に投与したテトロドトキシンの動態 (2012)
  • Author(s): 王俊杰,荒木泰一朗,辰野竜平,新名真也,池田光壱,高谷智裕,荒川修
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20120326-30