2013 Fiscal Year Final Research Report
Development of an isolation method of microsatellite DNA markers for evaluation of genetic diversity of organisms in rural areas
| Project/Area Number |
23580340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Irrigation, drainage and rural engineering/Rural planning
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
KOIZUMI Noriyuki 独立行政法人農業・食品産業技術総合研究機構, 農村工学研究所・資源循環工学研究領域, 主任研究員 (60301222)
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| Project Period (FY) |
2011 – 2013
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| Keywords | マイクロサテライトDNA / 遺伝子多様性 / 農村生態系 |
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| Research Abstract |
An isolation method of microsatellite DNA markers was developed for the evaluation of genetic diversity of organisms inhabiting rural areas. This method was composed of five processes such as DNA extraction, DNA fragmentation, screening of microsatellite regions, etc. Applying this method, nineteen to forty of novel microsatellite DNA markers were isolated and characterized for seven species of fish, dragonfly and mysid collected from agricultural canals. Furthermore, these markers were available for practical analysis of their genetic diversity and the structures of their populations. The development of the markers for other species based on this method and the investigation of genetic diversity using the developed markers are expected in the near future.
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Research Products
(14 results)
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[Presentation] Investigation of population genetic diversity of common small fish in the Mekong River Basin, Vientiane, Lao PDR, for conserving native fishes2013
Author(s)
Koizumi, N., Morioka, S., Mori, A.(他4名)
Organizer
Abstract book of 26th International Congress for Conservation Biology, 111-112
Place of Presentation
Baltimore, Maryland, USA
Year and Date
2013-07-22
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