2013 Fiscal Year Final Research Report
Basic study for development of a novel beef-fattening through control of brown adipocyte activity
| Project/Area Number |
23580368
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kyoto University |
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Principal Investigator |
FUNABA Masayuki 京都大学, (連合)農学研究科(研究院), 准教授 (40238655)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Tomoya 独立行政法人農業・食品産業技術総合研究機構, 研究員 (80343987)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 褐色脂肪細胞 / ウシ / 脂肪組織 / 転写因子 |
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| Research Abstract |
Uncoupling protein 1 (Ucp1), a gene specifically expressed in brown/beige adipocytes, was expressed in white adipose tissue of Japanese Black fattening steers. Expression level of Ucp1 was higher in subcutaneous fat depots of steers fed high concentrate diet than in those fed high roughage diet. In contrast, no significant differences were observed in Ucp1 expression in the mesenteric fat depots between the high concentrate diet group and the high roughage diet group. The cell culture studies revealed that insulin is essential for differentiation of brown preadipocytes to brown adipocytes, possibly through inducing Srebp1c, a transcription factor. Differentiation of brown preadipocytes was potently inhibited by the presence of TGF-beta 1 or activin A. Chronic treatment with thyroxine, rosiglitazone and isobuthylmethylxanthine induced differentiation toward Ucp1-expressing adipocytes in 3T3-L1 preadipocytes, a white preadipocyte line.
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