Study of regulation of anti-diuretic hormone gene expression

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580388
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Okayama University (2013); Nagoya University (2011-2012)
• Principal Investigator: SAITO Noboru 岡山大学, その他の研究科, 教授 (40211924)
• Co-Investigator(Renkei-kenkyūsha): HARADA Nobuhiro 藤田保険衛生大学, 医学部, 教授 (00189705)
• Project Period (FY): 2011 – 2013
• Keywords: ニワトリ / 浸透圧調節 / 抗利尿ホルモン / 遺伝子発現

Research Abstract

We suggested that Tonicity-responsive Enhancer Binding Protein (TonEBP) participated in expression of birds adjustment by the osmoregulation of arginine vasotocin (AVT) which was antidiuretic hormone. We revealed that TonEBP bound to the upper region of the AVT gene by the gel mobility shift assay method. In addition, an immunoreactive localization of TonEBP was observed in PVN (para-ventricular nucleus) and SON (supra optic nucleus) with many VT-positive cells. It was suggested that TRPV (Transient Receptor Potential Vanilloid) participated in the osmoregulation of birds.

Research Products

• [Journal Article] Vasotocin-mRNA expression is sensitive to testosterone and oestradiol in the bed nucleus of the stria terminalis in female Japanese quail (2013)
  • Author(s): Aste N, Sakamoto E, Kagami M, Saito N.
  • Journal Title: Journal of Neuroendocrinology Volume: 25 Pages: 811-825
  • DOI: 10.1111/jne.12076
  • Peer Reviewed
• [Journal Article] Analysis of egg white lysozyme polymorphisms among Japanese quail populations in Japan and France (2012)
  • Author(s): Myint S. L., Shimogiri T., Kinoshita K., Nirasawa K., Saitoh N., Watanabe H., Kawabe K., Maeda Y. and Okamoto S.
  • Journal Title: Journal of Poultry Science Volume: 49 Pages: 74-78
  • DOI: 10.2141/jpsa.011129
  • Peer Reviewed
• [Journal Article] Inheritance and developmental pattern of cerebral hernia in the crested polish chicken (2012)
  • Author(s): Yoshimura K, Kinoshita K, Mizutani M, Matsuda Y, Saito N.
  • Journal Title: Journal of Experimental Zoology Mol Dev Evol Volume: 318B Pages: 613-620
  • DOI: 10.1002/jez.b.22464
  • Peer Reviewed
• [Journal Article] The mechanism underlying the central glucagon-induced hyperglycemia and anorexia in chicks. Comparative Biochemistry and Physiology Part A (2012)
  • Author(s): Honda K, Kamisoyama H, Uemura T, Yanagi T, Saito N, Kurose Y, Sugahara K, Katoh K, Hasegawa S.
  • Journal Title: Molecular & Integrative Physiology Volume: 163 Pages: 260-264
  • DOI: 10.1016/j.cbpa.2012.08.005
  • Peer Reviewed
• [Journal Article] LIM-homeodomain transcription factor, Lhx2, is involved in transcriptional control of brain-specific promoter/exon 1f of the mouse aromatase gene (2012)
  • Author(s): Honda S-I, Kozako T, Shimeno H, Soeda S, Harada N.
  • Journal Title: J Neuroendocrinol Volume: 24 Pages: 1367-1374
  • DOI: 10.1111/j.1365-2826
  • Peer Reviewed
• [Journal Article] Brain endogenous estrogen levels determine responses to estrogen replacement therapy via regulation of BACE1 and NEP in female Alzheimer's transgenic mice (2012)
  • Author(s): Li R, He P, Cui J, Staufenbiel M, Harada N, Shen Y.
  • Journal Title: Mol Neurobiol
  • DOI: 10.1007/s12035-012-8377-3
  • Peer Reviewed
• [Journal Article] Aromatase expression in the brain of the ruffed grouse (Bonasa umbellus) and comparisons with other galliform birds (Aves, Galliformes) (2012)
  • Author(s): Cornfield JR, Harada N, Iwaniuk AN.
  • Journal Title: J Chem Neuroanat
  • DOI: 10.1016/j.jchemneu.2012.12.001
  • Peer Reviewed
• [Journal Article] Estradiol Promotes Purkinje Dendritic Growth, Spinogenesis, and Synaptogenesis During Neonatal Life by Inducing the Expression of BDNF (2012)
  • Author(s): Haraguchi, S., Sasahara, K., Shikimi, H., Honda, S. I., Harada, N., & Tsutsui, K.
  • Journal Title: Cerebellum Volume: 11 Pages: 416-417
  • DOI: 10.1007/s12311-011-0342-6
  • Peer Reviewed
• [Journal Article] Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle and gastro-intestinal tract of chicken (2011)
  • Author(s): Yoshimura K, Sugiura K, Ohmori Y, Aste N, Saito N.
  • Journal Title: Cell and Tissue Research Volume: 344 Pages: 51-61
  • DOI: 10.1007/s00441-011-1134-5
  • Peer Reviewed
• [Journal Article] Sex differences in brain aromatase activity : genomic and non-genomic controls (2011)
  • Author(s): Balthazart, J., Charlier, T.D., Cornil, C.A., Dickens, M.J., Harada, N., Konkle, A.T.M., Voigt, C., Ball, G.F.
  • Journal Title: Front. Neuroendocrin Volume: 2 Pages: 34
  • DOI: 10.3389/fendo.2011.00034,2011
  • Peer Reviewed
• [Journal Article] Behavioral analysis of genetically modified mice indicates essential roles of neurosteroidal estrogen (2011)
  • Author(s): Honda, S-I., Wakatsuki, T., & Harada, N.
  • Journal Title: Front. Neuroendocrin Volume: 2 Pages: 40
  • DOI: 10.3389/fendo.2011.00040
  • Peer Reviewed
• [Journal Article] 1-(2,4-Dihydroxyphenyl)-3-(2,4-dimethoxy-3- methylphenyl) propane inhibits melanin synthesis by dual mechanisms (2011)
  • Author(s): Niki, Y., Yoshida, M., Ando, H., Wakamatsu, K., Ito, S., Harada, N., Matsui, M.S., Yarosh, D.B., Ichihashi, M.
  • Journal Title: J. Dermatol. Sci Volume: 63 Pages: 115-121
  • DOI: 10.1016/j.jdermsci.2011.04.002
  • Peer Reviewed
• [Journal Article] Sex steroid hormones in pairs of tumor and serum from breast cancer patients and pathological role of androstene-3b, 17b-diol (2011)
  • Author(s): Honma, N., Saji, S., Hirose, M., Horiguchi, S., Kuroi, K., Hayashi, S., Utsumi, T., & Harada, N.
  • Journal Title: Cancer Sci Volume: 102 Pages: 1848-1854
  • DOI: 10.1111/j.1349-7006.2011.02018.x
  • Peer Reviewed
• [Journal Article] Human and Quail Aromatase Activity Is Rapidly and Reversibly Inhibited by Phosphorylating Conditions (2011)
  • Author(s): Charlier, T. D., Harada, N., Balthazart, J., & Cornil, C. A.
  • Journal Title: Endocrinology Volume: 152 Pages: 4199-4210
  • DOI: 10.1210/en.2011-0119
  • Peer Reviewed
• [Presentation] Cerebral hernia in the crested Polish chicken : inheritance and developmental pattern. Chick 7 (2012)
  • Author(s): Saito N., Yoshimura K., Kinoshita K., Mizutani M. and Matsuda Y.
  • Organizer: Avian Model Systems 7^<th> International Chick Meeting
  • Place of Presentation: 名古屋
  • Year and Date: 20121114-18
• [Presentation] Arginine-vasotocin (AVT)-mRNA expression is sensitive to testosterone and estradiol in the bed nucleus of the stria terminalis of adult female Japanese quail (2012)
  • Author(s): Aste N., Kagami M., Sakamoto E. and Saito N.
  • Organizer: 10^<th> International Symposium on Avian Endocrinology 2012
  • Place of Presentation: 岐阜
  • Year and Date: 20120605-09
• [Presentation] Arginine-vasotocin (AVT)-expression is female-biased and associated with aromatase in the bed nucleus of the stria terminalis of developing Japanese quail (2012)
  • Author(s): Aste N., Yoshioka N., Sakamoto E.and Saito N.
  • Organizer: 10^<th> International Symposium on Avian endocrinology 2012
  • Place of Presentation: 岐阜
  • Year and Date: 20120605-09
• [Presentation] TonEBP mediates the effects of hyperosmolality on arginine vasotocin gene expression in the hypothalamus of chick (Gallus domesticus) (2012)
  • Author(s): Saito N., Nozawa S., Fujii M. and Aste N.
  • Organizer: 10^<th> International Symposium on Avian endocrinology 2012
  • Place of Presentation: 岐阜
  • Year and Date: 20120605-09
• [Presentation] Polish系ニワトリにおける"Cerebral hernia"形成機構の解明 (2011)
  • Author(s): 吉村圭司、木下圭司、水谷誠、松田洋一、齋藤昇
  • Organizer: 鳥類内分泌研究会
  • Place of Presentation: 箱根町
  • Year and Date: 20111121-22
• [Presentation] Effects of fasting on AQP9 expression in the chick brain (2011)
  • Author(s): Yoshimura K, Aste N, Saito N.
  • Organizer: International Congress of Comparative Physiology and Biochemistry 2011
  • Place of Presentation: 名古屋
  • Year and Date: 20110531-0605
• [Presentation] Arginine-vasotocin (AVT) mRNA expression is sensitive to testosterone and estradiol in adult female Japanese quail (2011)
  • Author(s): Aste N, Sakamoto E, Kagami M, Saito N.
  • Organizer: International Congress of Comparative Physiology and Biochemistry 2011
  • Place of Presentation: 名古屋
  • Year and Date: 20110531-0605
• [Presentation] Muscular dystrophic chicken is hypernatremic (2011)
  • Author(s): Saito N, Hirayama H, Yoshimura K, Atsumi Y, Kinoshita K, Mizutani M, Fujiwara A, Namikawa T, Nicoletta Aste N.
  • Organizer: International Congress of Comparative Physiology and Biochemistry 2011
  • Place of Presentation: 名古屋
  • Year and Date: 20110531-0605