2013 Fiscal Year Final Research Report

Basic research for resurrection of extinct animals by somatic cell nuclear transfer

Research Project

Project/Area Number	23580395
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied animal science
Research Institution	Kinki University
Principal Investigator	KATO Hiromi 近畿大学, 先端技術総合研究所, 教授 (60330320)
Project Period (FY)	2011 – 2013
Keywords	クローン動物 / 絶滅動物 / 異種間核移植
Research Abstract	The analysis of the conservation state of existing mammoth soft tissue (skin, muscle and bone marrow) by PCR and the development of the method to collect somatic cell nuclei from bone marrow were examined. In results, we succeeded to develop the efficient method to collect somatic cell nuclei from bone marrow. Also we succeeded to have new, well conserved mammoth's skin, muscle, hypodermis and bone marrow from Sakha (Yakutia) Republic. Furthermore, to improve developmental ability of reconstructed embryos, we tried to co-inject mitochondria which derived from cells of same species with donor cell nucleus. However the developmental ability of reconstructed embryos was not improved.

(2 results)