2013 Fiscal Year Final Research Report

Effects of metabolism of purine derivatives on bovine embryo development

Research Project

Project/Area Number	23580398
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied animal science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	KIMURA Koji 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所家畜飼養技術研究領域, 主任研究員 (50355070)
Project Period (FY)	2011 – 2013
Keywords	ウシ / 胚 / 代謝 / プリン誘導体
Research Abstract	The effects of metabolism of purine derivatives on bovine embryo development were investigated. When de novo purine synthesis was inhibited, the embryo development after maternal-to-zygotic transition (MZT) was severely compromised. Moreover, when the purine derivatives (adenine or hypoxanthine) were supplemented in the presence of the inhibitor for de novo purine synthesis, the inhibitory effect disappeared. These results suggested that purines are necessary for bovine embryo development after MZT. However, excess amount of purine derivatives (especially adenine) deteriorated bovine embryo development. In the presence of excess amount of adenine after fertilization, the cleavage of the embryo was arrested and the fragmentation of nuclear was induced.