2013 Fiscal Year Final Research Report
The expression of Glutamate-associate molecular and neurotrophic factor in the cochlea.
| Project/Area Number |
23580401
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Basic veterinary science/Basic zootechnical science
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ATOJI Yasuro 岐阜大学, 応用生物科学部, 教授 (90151084)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 蝸牛 / グルタミン酸 / グルタミン酸トランスポーター / グルタミン酸レセプター / 神経栄養因子 / プロサポシン / In situ hybridization / RT-PCR |
|---|
| Research Abstract |
The auditory stimulus is detected by inner hair cells and then transmitted to the brain by type I spinal ganglion neurons. In mammals, type I spinal ganglion neurons are revealed to express ionotropic AMPA, NMDA and kainate type receptors to form excitatory synapses with inner hair cells, and also appear to express vesicular glutamate transporter I to release glutamate to neurons in the brain. In birds, receptor neurons and spinal ganglion neurons could not be subdivided as mammals. Among receptor subunits examined, GluA1, 2, 3 and 4 mRNAs for AMPA type, GluN1, 2A, 2B and 3A for NMDA type, and GluK1, 2 and 4 mRNAs for kainate type were observed. Vesicular glutamate transporter II and III were also identified with different expression levels in the basilar papilla, and the spinal ganglion neurons were revealed to express vesicular glutamate transporter II. This study revealed glutamate plays a pivotal role in the excitatory synapse transmission in avian auditory pathway.
|
