Clarification of the natural host defense system against indigenous bacteria in alimentary tract

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kobe University
• Principal Investigator: KITAGAWA Hiroshi 神戸大学, 自然科学系先端融合研究環, 教授 (40125307)
• Co-Investigator(Kenkyū-buntansha): KAWANO Junichi 神戸大学, (連合)農学研究科(研究院), 教授 (40127361)
• Project Period (FY): 2011 – 2013
• Keywords: 生体防御機構 / 消化管 / 常在細菌 / 自然免疫 / Toll-like receptor / 免疫組織化学

Research Abstract

The research achievements on the natural host defense against indigenous bacteria (IB) in animals is as follows: the clarification of the relationship between IB settlement and membranous Toll-like receptor (mTLRs)/secretory TLRs (sTLRs) in small and large intestines, the clarification of the relationship between the alternation of IB settlement and the bactericidal substance secretion in small intestine, the clarification of the peculiar epithelial differentiation in the follicle-associated intestinal crypts of gut-associated lymphatic tissue, the clarification of the detailed host defense with epithelial apoptosis against IB hyperproliferation in esophagus and epidermis, the demonstration of the direct transportation of chylomicrons from the intestinal epithelium into the hepatic portal blood and the endothelial receptor for chylomicron transportation, the clarification of the recognition system with mTLRs and secretion of sTLRs against IB in the respiratory tract.

Research Products

• [Journal Article] Ultrastructural and histochemical study on the epithelial differentiation to Paneth cells in the rat ascending colon (2014)
  • Author(s): Mantani, Y., Nishida, M., Yuasa, H., Yamamoto, K., Takahara, E., Omotehara, T., Udayanga, K. G. S., Kawano, J., Yokoyama, T., Hoshi, N. and Kitagawa, H.
  • Journal Title: Anatomical Record Volume: (accepted)
  • DOI: 10.1002/ar.22937
  • Peer Reviewed
• [Journal Article] Peculiar composition of epithelial cells in follicle-associated intestinal crypts of Peyer's patches in the rat small intestine (2014)
  • Author(s): Mantani, Y., Yuasa, H., Nishida, M., Takahara, E., Omotehara, T., Udayanga, K. G. S., Kawano, J., Yokoyama, T., Hoshi, N. and Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 76(in press) Pages: 83-838
  • DOI: 10.1292/jvms.14-0026
  • Peer Reviewed
• [Journal Article] Ultrastructural demonstration of the absorption and transportation of minute chylomicrons by subepithelial blood capillaries in rat jejunal villi (2013)
  • Author(s): Takahara, E., Mantani, Y., Udayanga, K. G. S., Qi, W.-M., Tanida, T., Takeuchi T., Yokoyama, T., Hoshi, N. and Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 75 Pages: 1563-1569
  • DOI: 10.1292/jvms.13-0310
  • Peer Reviewed
• [Journal Article] Histoplanimetrical study on the relationship between invasion of indigenous bacteria into intestinal crypts and proliferation of epithelial cells in rat ascending colon (2013)
  • Author(s): Mantani, Y., Takahara, E., Takeuchi, T., Kawano, J., Yokoyama, T., Hoshi, N. and Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 75 Pages: 939-947
  • DOI: 10.1292/jvms.13-0036
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of the Toll-like receptor-2, -4 and -9 in exocrine glands associated with rat alimentary tract (2012)
  • Author(s): Mantani, Y., Yokoo, Y., Kamezaki, A., Udayanga, K. G. S., Takahara, E., Takeuchi, T., Kawano, J., Yokoyama, T., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 74 Pages: 1429-1438
  • DOI: 10.1292/jvms.12-0146
  • Peer Reviewed
• [Journal Article] Persorption of IgG-Fc-coated particulates form intestinal lumen into portal blood via villous columnar epithelial cells in rat small intestine (2012)
  • Author(s): Yuji, M., Fujimoto, M., Qi, W.-M., Takahara, E., Takahara, E., Udayanga, K. G. S., Takeuchi, T., Warita, K., Yokoyama, T., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 74 Pages: 1447-1452
  • DOI: 10.1292/jvms.12-0111
  • Peer Reviewed
• [Journal Article] Alteration in the apoptosis process of rat esophageal epithelium with hyperproliferation of indigenous bacteria un a physiological condition (2012)
  • Author(s): Udayanga, K. G. S., Yamamoto, K., Miyata, H., Yokoo, Y., Mantani, Y., Takahara, E., Qi, W.-M., Kawano, J., Yokoyama, T., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 74 Pages: 597-605
  • DOI: 10.1292/jvms.11.0516
  • Peer Reviewed
• [Journal Article] Immunohistochemical study on the secretory host defense system of bactericidal peptides in rat digestive organs (2011)
  • Author(s): Yokoo, Y., Miyata, H., Udayanga, K. G. S., Qi, W.-M., Takahara, E., Yokoyama, T., Kawano, J., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 73 Pages: 217-225
  • DOI: 10.1292/jvms.10-0293
  • Peer Reviewed
• [Journal Article] Immunohistochemical and histoplanimetrical study on the spatial relationship between the settlement of indigenous bacteria and the secretion of bactericidal peptides in rat alimentary tract (2011)
  • Author(s): Yokoo, Y., Miyata, H., Udayanga, K. G. S., Qi, W.-M., Takahara, E., Mantani, Y., Yokoyama, T., Kawano, J., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 73 Pages: 1043-1050
  • DOI: 10.1292/jvms.11-0114
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of phosphatidylserine and thrombospondin on the denucleating erythroblasts in rat bone marrow (2011)
  • Author(s): Yokoyama, T., Etoh, T., Udayanga, K. G. S., Qi, W.-M., Warita, K., Matsumoto, Y., Miki, T., Takeuchi, Y., Hoshi, N., Kitagawa, H.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 73 Pages: 949-952
  • DOI: 10.1292/jvms.10-0437
  • Peer Reviewed
• [Journal Article] Site differences of the Toll-like receptor expression in the mucous epithelium of rat small intestine (2011)
  • Author(s): Mantani, Y., Kamezaki, A., Udayanga, K. G. S., Takahara, E., Qi, W.-M., Yokoyama, T., Kawano, J., Hoshi, N., Kitagawa, H.
  • Journal Title: Histology and Histopathology Volume: 26 Pages: 1295-1303
  • URL: http://www.hh.um.es/Abstracts/Vol_26/26_10/26_10_1295.htm
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition (2011)
  • Author(s): Udayanga, K. G. S., Miyata, H., Yokoo, Y., Qi, W.-M., Takahara, E., Mantani, Y., Yokoyama, T., Hoshi, N., Kitagawa, H.
  • Journal Title: Histology and Histopathology Volume: 26 Pages: 811-820
  • URL: http://www.hh.um.es/Abstracts/Vol_26/26_7/26_7_811.htm
  • Peer Reviewed
• [Presentation] ラットの消化器系における補体成分C3とC4の産生部位に関する免疫組織化学的研究 (2013)
  • Author(s): 又吉緑,万谷洋平,高原英一郎,横山俊史,河野潤一,星信彦,北川浩
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜市
  • Year and Date: 20130920-22
• [Presentation] ラット上行結腸の集合リンパ小節近傍の腸陰窩に出現するパネート細胞様外分泌細胞に関する超微形態学的および組織化学的研究 (2013)
  • Author(s): 万谷洋平,高原英一郎,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜市
  • Year and Date: 20130920-22
• [Presentation] ラット空腸の腸絨毛における小型乳ビ球の門脈血への移行の仕組みに関する免疫組織化学的研究 (2013)
  • Author(s): 高原英一郎,万谷洋平,横山俊史,星信彦,北川浩
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜市
  • Year and Date: 20130920-22
• [Presentation] ラット上行結腸における酸好性顆粒を有する腸陰窩上皮胞に関する形態学的研究 (2013)
  • Author(s): 万谷洋平,高原英一郎,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第89回日本解剖学会近畿支部学術集会
  • Place of Presentation: 生駒市
  • Year and Date: 2013-11-30
• [Presentation] ラット小腸腸陰窩上皮細胞の細胞死に関する超微形態学的および免疫組織化学的研究 (2012)
  • Author(s): 山本教司,樅山真丈,Udayanga K. G. S.,万谷洋平,高原英一郎,竹内崇師,横山俊史,河野潤一,星信彦,北川浩
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手市
  • Year and Date: 20120914-16
• [Presentation] ラットの上行結腸における常在細菌の定着とその制御に関する定量組織学的研究 (2012)
  • Author(s): 万谷洋平,高原英一郎,竹内崇師,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手市
  • Year and Date: 20120914-16
• [Presentation] ラット上行結腸腸陰窩における常在細菌の増殖に対する生体防御に関する研究 (2012)
  • Author(s): 万谷洋平,高原英一郎,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第88回日本解剖学会近畿支部学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-01
• [Presentation] ラット肝臓における補体C3およびC4の産生に関する免疫組織化学的研究 (2012)
  • Author(s): 又吉緑,万谷洋平,高原英一郎,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第88回日本解剖学会近畿支部学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-01
• [Presentation] ラット十二指腸粘膜上皮におけるMyeloid differentiation factor-2とToll-like receptor-2の発現に関する免疫組織化学的研究 (2012)
  • Author(s): 樅山真丈,万谷洋平,高原英一郎,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第88回日本解剖学会近畿支部学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-01
• [Presentation] Mitochondria-Dependent Pathway as a Final Apoptosis Process Secondarily Induced in Rat (2011)
  • Author(s): Udayanga K. G. S., Miyata H., Yokoo Y., Takahara E., Mantani Y., Yokoyama T., Hoshi N., Kitagawa H.
  • Organizer: Esophageal Epithelium under a Physiological Condition. Mitochondria, Apoptosis and Cancer Conference
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 20111027-29
• [Presentation] Acceleration of epithelial apoptosis in anterior epithelial column of filiform papilla in rat tongue by indigenous bacterial settlement (2011)
  • Author(s): Udayanga K. G. S., Yamamoto K., Mantani Y., Takahara E., Tanida T., Yokoyama T., Kawano J., Hoshi N., Kitagawa H.
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 堺市
  • Year and Date: 20110919-21
• [Presentation] ラット呼吸器系上皮におけるToll-like receptorの局在に関する免疫組織化学的研究 (2011)
  • Author(s): 亀崎青沙,万谷洋平,Udayanga K. G. S.,高原英一郎,谷田任司,河野潤一,横山俊史,星信彦,北川浩
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 堺市
  • Year and Date: 20110919-21