2013 Fiscal Year Final Research Report
Functional analysis of tubulin acetylation during mouse preimplantation development
| Project/Area Number |
23580416
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Basic veterinary science/Basic zootechnical science
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 発生 / アセチル化 |
|---|
| Research Abstract |
Histone Deacetylases (HDACs) are known to play important roles during preimplantation development. However, the roles of acetylation of non-histone proteins mouse embryos still remains poorly understood. In this study, we have focused on acetylation of alpha-tubulin and HDACs in terms of oocyte quality during one-cell stage. First, we found that treatment with NAM, nicotinamide, an inhibitor for Class III HDACs inhibited cellular fragmentation and spindle elongation after postovulatory in vitro aging. Also, the alpha-tubulin increased acetylation during aging, suggesting not only histone but non-histone protein acetylation also increases with oocyte aging. Further, once an oocyte has been activated, histone and nonhistone proteins are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation. Thus, we provide the evidence that protein acetylation play important roles for oocyte quality which affect subsequent embryonic development.
|
Research Products
(13 results)
-
-
-
-
-
[Journal Article] Successful serial recloning in the mouse over multiple generations2013
Author(s)
Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T.
-
Journal Title
Cell Stem Cell
Volume: 12
Pages: 293-297
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
