2013 Fiscal Year Final Research Report
Development of next-generation dengue virus vaccine
| Project/Area Number |
23580422
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Osaka University (2012-2013)
Kyoto University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | デングウイルス / ワクチン |
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| Research Abstract |
To develop a new DNA vaccine for dengue virus (DV) infection, we have constructed a plasmid-based infectious clone encoding full-length DV-1 cDNA under control of the minimum cytomegalovirus promoter. In our experiments, yields of WT virus 24 hours after plasmid transfection are regularly in the range of 100 to 1000 PFU/ml. Furthermore, DV-1 reporter replicon with luciferase was made by replacing part of the DV-1 structural gene region with the luciferase gene.
To improve the reverse genetics for DV, we have developed a homologous recombination-based reverse genetics for DV. Furthermore, we have rescued a chimeric DV, which expresses the prM and E genes from DV type 2 in a DV type 1 genetic background.
These improved infectious cDNA clone and reporter replicon systems described here can be employed for studies of the DV replication and pathogenesis and used to develop vaccines, diagnostics, and therapeutics.
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[Journal Article] Imported case of acute respiratory tract infection associated with a member of species Nelson Bay orthoreovirus2014
Author(s)
Yamanaka A., Iwakiri A., Yoshikawa T., Sakai K., Harpal S., Himeji D., Kikuchi I., Ueda1 A., Yamamoto S., Miura M., Shioyama Y., Kawano K., Nagaishi T., Saito M., Minomo M., Iwamoto N., Hidaka Y., Sohma H., Kobayashi T., Kanai Y., Kawagishi T., Nagata N., Fukushi S., Mizutani T., Tani H., Taniguchi S., Fukuma A., Shimojima M., Kurane I., Kageyama T., Odagiri T., Saijo M., and Morikawa S.
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Journal Title
PLoS One
Volume: 9
Pages: e92777
Peer Reviewed
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