The mode of infection of anti-microbial resistant Salmonella and the transmission of the determinants of the resistance.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580426
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Kagoshima University
• Principal Investigator: CHUMA Takehisa 鹿児島大学, 獣医学部, 教授 (90201631)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Karoku 鹿児島大学, 共同獣医学部, 教授 (00136847)
• Project Period (FY): 2011 – 2013
• Keywords: サルモネラ / 抗菌剤耐性 / ブロイラー

Research Abstract

The study was conducted to determine the antimicrobial resistance and characterize the B-lactamase genes and the plasmids in S. Infantis isolates from broilers. In 93 (6.3%) isolates recovered, 33 (35.5%) isolates showed resistance to cefotaxime conferred by TEM-20, TEM-52 and CTX-M-25 ESBLs. In addition to ESC-resistance, eight (8.6%) isolates exhibited resistance to cefoxitin mediated by CMY-2 AmpC B-lactamase. Plasmid analysis revealed the blaTEM-20 and blaCMY2 genes were associated with IncP plasmids, blaTEM was -52 linked with a non-typable plasmid and blaCTX-M-25 was carried by an IncA/C plasmid. Resistance to streptomycin, sulfamethoxazole, and oxytetracycline encoded by the aadA1, sul1, and tetA, respectively, was found in 86 (92.5%) isolates. These data indicate that S. Infantis isolates producing ESBLs and AmpC B-lactamase have spread among broiler farms in Japan. These data demonstrated the incidence of ESC-resistant S. Infantis carrying blaTEM-52 remarkably increased.

Research Products

• [Journal Article] Chronological Change of Resistance to β-Lactams in Salmonella enterica serovar Infantis Isolated from Broilers in Japan (2013)
  • Author(s): Chuma T, Miyasako D, Dahshan H, Takayama T, Nakamoto Y, Shahada F, Akiba M, and Okamoto K.
  • Journal Title: Front. Microbiol Volume: 4 Pages: 1-5
• [Journal Article] Prevalence and Epidemiological Relationship of CMY-2 AmpC β-Lactamase and CTX-M Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Broiler Farms in Japan (2013)
  • Author(s): Kameyama M, Chuma T, Yabata J, Tominaga K, Iwata H, Okamoto K.
  • Journal Title: J. Vet. Med. Sci Volume: 75 Pages: 1009-1015
• [Journal Article] Distribution of extended-spectrum cephalosporin resistance determinants in Salmonella enterica and Escherichia coli isolated from broilers in southern Japan (2013)
  • Author(s): Shahada F, Chuma T, Kosugi G, Kusumoto M, Iwata T, Akiba M.
  • Journal Title: Poult. Sci Volume: 92 Pages: 1641-1649
• [Journal Article] Emergence of Salmonella enterica serovar Infantis harboring IncI1 plasmid with blaCTX-M-14 in a broiler farm in Japan (2012)
  • Author(s): Kameyama M, Chuma T, Yokoi T, Yabata J, Tominaga K, Miyasako D, Iwata H, and Okamoto K.
  • Journal Title: J. Vet. Med. Sci Volume: 74 Pages: 1213-1216
• [Presentation] ブロイラー由来サルモネラから検出された薬剤耐性遺伝子保有プラスミドの特徴と分布 (2014)
  • Author(s): 中馬猛久、野尻彩歌、永松大和、安藤匡子
  • Organizer: 第87回日本細菌学会
  • Place of Presentation: 船堀ホール 東京
  • Year and Date: 20140000
• [Presentation] ブロイラー由来サルモネラにおける薬剤耐性獲得とプラスミドの関係 (2013)
  • Author(s): 野尻彩歌、永松大和、中馬猛久
  • Organizer: 第156回日本獣医学会
  • Place of Presentation: 岐阜大学 岐阜
  • Year and Date: 20130000
• [Presentation] ブロイラー農場におけるESBL、AmpC 型β-ラクタマーゼ産生大腸菌の分布 (2013)
  • Author(s): 亀山光博、中馬猛久、矢端順子、富永潔、岩田祐之、岡本嘉六
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学 東京
  • Year and Date: 20130000
• [Presentation] ブロイラーから分離されたサルモネラと大腸菌における広域セファロスポリン耐性因子の分布 (2012)
  • Author(s): Francis Shahada、中馬猛久、小杉岳童、楠本正博、岩田剛敏、秋庭正人
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学盛岡
  • Year and Date: 20120000
• [Presentation] ブロイラーから分離されたSalmonella Infantis の薬剤感受性とβ ラクタマーゼ産生型の推移 (2012)
  • Author(s): 中馬猛久、宮迫大輔、Hesham Dahshan、横井智毅、仲本佑子、岡本嘉六
  • Organizer: 第153回日本獣医学会
  • Place of Presentation: 大宮ソニックシティ 大宮
  • Year and Date: 20120000
• [Presentation] Chronological change of resistance to β-lactams in Salmonella enterica serovar Infantis isolated from broilers in Japan (2012)
  • Author(s): T. Chuma, D. Miyasako, H. Dahshan, T.Takayama, Y. Nakamoto, F. Shahada, M. Akiba, K. Okamoto
  • Organizer: 3rd ASM Conference on antimicrobial resistance in zoonotic bacteria and foodborne pathogens in animals, humans, and the environment
  • Place of Presentation: Aix-en Provence, France
  • Year and Date: 2012-06-26
• [Presentation] Distribution of extended-spectrum cephalosporin resistance determinants of Salmonella enterica and Escherichia coli isolated from broilers in soutern Japan (2012)
  • Author(s): F. Shahada, G. Kosugi, M. Kusumoto, T. Iwata, T. Chuma, M. Akiba
  • Organizer: 3rd ASM Conference on antimicrobial resistance in zoonotic bacteria and foodborne pathogens in animals, humans, and the environment
  • Place of Presentation: Aix-en Provence, France
  • Year and Date: 2012-06-26