2013 Fiscal Year Final Research Report
Corneal Regeneration Therapy with limbal epithelial cell sheet cultivated on amniotic membrane in dogs
| Project/Area Number |
23580437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUZUKI Keiko 東京大学, 農学生命科学研究科, 特任助教 (30364251)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 再生医療 / 角膜上皮シート / 角膜損傷 |
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| Research Abstract |
Canine corneal epithelial cell sheets were successfully cultivated on canine amniotic membrane. Further investigation revealed that more immature corneal epithelial cells were sustained in epithelial cell sheets cultivated on atelocollagen substrate. Transplantation to canine corneal injury model was performed and reduction of corneal opacity was observed in transplanted group. Additionally, histopathological evaluation revealed that transplanted corneal epithelial cell sheet successfully exhibited K3 (differentiated corneal epithelial cell marker) and p63 (immature marker of corneal epithelial cells). However, hypercellularity of transplanted corneal epithelial cells were observed and more long-term investigation on safety and efficacy of transplantation of corneal cell sheet should be needed for clinical application in the future.
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[Journal Article] Evaluation of ABCG2 and p63 expression in canine cornea and cultivated corneal epithelial cells2014
Author(s)
Maresuke M., Fujita N., Takahashi A., Nam ER., Yui S., Chung CS., Kawahara N., Lin HY., Tsuzuki K., Nakagawa T., Nishimura R.
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Journal Title
Veterinary Ophalmology
Volume: (in press)
DOI
Peer Reviewed
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