2013 Fiscal Year Final Research Report
Study on molecular basis of growth factor/receptor of canine hemangiosarcoma
| Project/Area Number |
23580439
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Clinical veterinary science
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 血管肉腫 / 犬 / 培養細胞株 / mTOR / PDGFR |
|---|
| Research Abstract |
Seven canine hemangiosarcoma cell lines were established for analysis of pathogenesis of hemangiosarcoma. All cell lines showed active intake of DiI-Ac-LDL, and positive for CD31, VEGF-A, bFGF, HGF, IGF-I and PDGF-B mRNA by RT-PCR. Constitutive activation of mTORC2/Akt/4E-BP1 associated with the proliferation of cell lines in vitro and in vivo, and this activation was confirmed in spontaneous canine hemangiosarcomas. Moreover, the high expression of PDGF receptor beta without genomic mutation of jaxutamembrane area was detected in hemangiosarcoma. It is suggested that mTOR and PDGF receptor beta may be considered as one of therapeutic targets.
|
Research Products
(25 results)
-
[Journal Article] Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours2014
Author(s)
Abou Asa, S., Mori, T., Maruo, K., Khater, A., El-Sawa,k A., Abd El-Aziz, E., Yanai, T., and Sakai, H.
-
Journal Title
Vet. Comp. Oncol
Volume: (in press)
Peer Reviewed
-
-
-
[Journal Article] Chemically Modified Synthetic microRNA-205 Inhibits the Growth of Melanoma Cells In Vitro and In Vivo2013
Author(s)
Noguchi, S., Iwasaki, J., Kumazaki, M., Mori, T., Maruo, K., Saka,i H., Yamada, N., Shimada, K., Naoe, T., Kitade, Y., and Akao, Y.
-
Journal Title
Mol. Ther
Volume: 21(6)
Pages: 1204-11
Peer Reviewed
-
[Journal Article] The Class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma2013
Author(s)
Kato, Y., Murakami, M., Hoshino, Y., Mori, T., Maruo, K., Hirata, A., Nakagawa, T. L. D. R., Yanai, T., and Sakai, H.
-
Journal Title
J. Comp. Pathol
Volume: 148
Pages: 188-96
Peer Reviewed
-
[Journal Article] ImmunohistochemicalAnalysis of the Akt/mTOR/4E-BP1 signalling pathway in canine haemangiomas and haemangiosarcomas2012
Author(s)
Murai, A., Abou, A. S., Kodama, A., Sakai, H., Hirata, A., Yanai, T.
-
Journal Title
J. Comp. Pathol
Volume: 147
Pages: 430-440
Peer Reviewed
-
[Journal Article] Fascin-1 expression in canine cutaneous and oral melanocytic tumours2012
Author(s)
Yamada, N., Mori, T., Murakami, M., Noguchi, S., Sakai, H., Akao, Y., Maruo, K.
-
Journal Title
Vet. Comp. Oncol
Volume: 10
Pages: 303-311
Peer Reviewed
-
[Journal Article] Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell lines2012
Author(s)
Murai, A., Asa, S. A., Kodama, A., Hirata, A., Yanai, T., and Sakai, H.
-
Journal Title
BMC Vet. Res
Volume: 8
Pages: 128
Peer Reviewed
-
[Journal Article] Cytologically atypical anal sac gland carcinoma in a dog2012
Author(s)
Sakai, H., Murakami, M., Mishima, H., Hoshino, Y., Mori, T., Maruo, K. and Yanai, T.
-
Journal Title
Vet. Clin. Pathol
Volume: 41
Pages: 291-294
Peer Reviewed
-
-
[Journal Article] Expression of platelet-derived growth factor and its receptors in spontaneous canine hemangiosarcoma and cutaneous hemangioma2012
Author(s)
Abou Asa, S., Murai, A., Murakami, M., Hoshino, Y., Mori, T., Maruo, K., Khater, A., El-sawak, A., Abd el-Aziz, E., Yanai, T., and Sakai, H.
-
Journal Title
Histol. Histopathol
Volume: 27
Pages: 601-607
Peer Reviewed
-
-
[Journal Article] Ganglioneuroma in the urinary bladder of a dog2011
Author(s)
Sakai, H., Yonemaru, K., Takeda, M., Niimi, K., Murakami, M., Hirata, A., and Yanai, T.
-
Journal Title
J. Vet. Med. Sci
Volume: 73
Pages: 801-803
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
