2014 Fiscal Year Final Research Report
Development of an effective laccase gene expression system by the edible basidiomycete Lentinula eodes
| Project/Area Number |
23580454
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 食用担子菌 / シイタケ / ラッカーゼ / プロトプラスト / 発現ベクター / 遺伝子発現 |
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| Outline of Final Research Achievements |
To develop an effective useful protein expression system by edible basidiomycetous fungi, improvements of Lentinula edodes transfomation method and laccase gene expression in L. edodes were studied. As a result, we were able to simplify the protoplast preparation method. Further, as a result of L. edodes transformation by laccase gene expression vector pChG-clcc1, a transformant exhibited more than two times higher laccase activity compared to the host was obtained. In addition, we isolated a laccase gene of lacquer (Toxicodendron vernicifluum) as a heterologous laccase gene for expression by L. edodes, and sequenced the full length of the laccase cDNA.
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| Free Research Field |
応用微生物学
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