Toward the understanding of the regulation of seed shattering habit in cereal plants using wild species

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kobe University
• Principal Investigator: ISHIKAWA RYO 神戸大学, (連合)農学研究科(研究院), 助教 (70467687)
• Co-Investigator(Renkei-kenkyūsha): TAKASHIGE Ishii 神戸大学, 大学院・農学研究科, 教授 (20260648)
• Project Period (FY): 2011 – 2013
• Keywords: イネ / 脱粒性

Research Abstract

Previously, two major quantitative trait loci (QTLs, qSH1 and sh4) were shown to be responsible for the seed-shattering degree. We produced introgression lines carrying cultivated alleles from O. sativa Nipponbare at the two major QTLs in wild rice O. rufipogon W630, genetic background. The introgression lines carrying Nipponbare alleles at either or both loci showed complete or partial abscission layer formation, respectively. This result indicated that other unknown loci might be involved in enhancing seed shattering in wild rice. Using an F2 population between Nipponbare and the wild introgression line carrying the Nipponbare alleles at the two QTLs. We identified a single QTL regulating seed-shattering degree and named it qSH3. A significant effect on seed-shattering degree was observed for the wild introgression line carrying Nipponbare alleles at qSH3 and the two QTLs, suggesting an important role of qSH3 on seed shattering in coordination with the two QTLs.

Research Products

• [Journal Article] Effect of quantitative trait loci for seed shattering on abscission layer formation in Asian wild rice Oryza rufipogon (2014)
  • Author(s): Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, and Ryo Ishikawa
  • Journal Title: Breeding Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] OsLG1 regulates a closed panicle trait in domesticated rice (2013)
  • Author(s): Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Norio Komeda, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, and Motoyuki Ashikari
  • Journal Title: Nature Genetics Volume: 45 Pages: 462-465
  • DOI: 10.1038/ng.2567
  • Peer Reviewed
• [Journal Article] Estimation of the outcrossing rate of Asian wild rice, Oryza rufipogon, under field conditions (2012)
  • Author(s): Phuong Dang Thai Phan, Hiroki Kageyama, Ryo Ishikawa, and Takashige Ishii
  • Journal Title: Breeding Science Volume: 62 Pages: 256-262
  • DOI: 10.1270/jsbbs.62.256
  • Peer Reviewed
• [Journal Article] Temporal changes of seed shattering degree of substitution lines having non-shattering alleles from cultivated rice (Oryza sativa) in the genetic background of wild rice (O. rufipogon) (2011)
  • Author(s): Than Myint Htun, Takashige Ishii, and Ryo Ishikawa
  • Journal Title: Journal of Crop research Volume: 56 Pages: 39-44
  • URL: http://ci.nii.ac.jp/naid/110008711270
  • Peer Reviewed
• [Presentation] 生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用 (2014)
  • Author(s): 井上千鶴, Than Myint Htun,石井尊生,石川亮野
  • Organizer: 日本育種学会第125回講演会
  • Place of Presentation: 東北大学
  • Year and Date: 20140321-22
• [Presentation] Identification of a quantitative trait locus involved in abscission layer formation for seed shattering in Asian wild rice, Oryza rufipogon (2014)
  • Author(s): Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, and Ryo Ishikawa
  • Organizer: 日本育種学会第125回講演会
  • Place of Presentation: 東北大学
  • Year and Date: 20140321-22
• [Presentation] Closed panicles in rice domestication (2013)
  • Author(s): Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, and Motoyuki Ashikari
  • Organizer: 7^<th> International Conference of Rice Genetics
  • Place of Presentation: マニラフィリピン
  • Year and Date: 20131105-08
• [Presentation] A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L (2013)
  • Author(s): Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, and Takashige Ishii
  • Organizer: 7^<th> International Conference of Rice Genetics
  • Place of Presentation: マニラフィリピン
  • Year and Date: 20131105-08
• [Presentation] 生穂が閉じることによって野生イネはどう変化したか? (2013)
  • Author(s): 沼口孝司, Htun Than Myint, Thanh Pham Thien,石川亮,石井尊
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 20131012-13
• [Remarks] 研究室ホームページ
  • URL: http://www.ans.kobe-u.ac.jp/kenkyuuka08/sigen/shokubutuikusyu.html